chemical

κ-Conotoxin RIIIK

STC-650

STC-650_0.1 mg

κ-Conotoxin RIIIK

Non Antibodies

Proteins

P69769

https://www.uniprot.org/uniprotkb/P69769/entry

Electrophysiology

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Conus radiatus (Rayed cone)

Synthetic peptide

KCNA2

KV1.2 is a mammalian voltage-dependent K+ channel, homologous to the Drosophila Shaker K+ channel. KV1.2 was first cloned from rat brain1. Eight Shaker related genes exist in mammals constituting the KV1, subfamily of the large KV channel family of genes2.The channel is expressed in neurons and cardiac and smooth muscle tissue as well as in retina and pancreas2. The crystal structure of KV1.2 was recently solved shedding light on the structure of a mammalian voltage-dependent channel3. The functional channel is considered low voltage activated and shows very little inactivation. Therefore, this channel activity influences the membrane potential and excitability of neurons and muscle.KV1.2 channels are sensitive to high doses of TEA (560 mM) and low doses of 4-AP (0.59 mM), the "classical" non-selective potassium channel blockers.Conotoxins, which are peptides consisting of 10 to 30 amino acid residues, typically have one or more disulfide bonds. Conotoxins have a variety of mechanisms of actions, most of which have not been determined. However, it appears that many of these peptides modulate the activity of ion channels4.κ-Conotoxin RIIIK is a 24 amino acid peptidyl toxin isolated from the Conus radiatus (Rayed cone) venoM It blocks Shaker and TSha1 K+ channels. Additionally, it was found that κM-conotoxin RIIIK blocks voltage-activated K+ channels with a novel pharmacophore that does not comprise a dyad motif5.

Alomone Labs

KV1.2 K+ channels

A Specific Potent Blocker of KV1.2 K+ Channels

A Specific Potent Blocker of KV1.2 K+ Channels

2649 Da

Kappa-M-RIIIK, Kappa- Conotoxin RIIIK, Conotoxin R3.1

Disulfide bonds between: Cys4-Cys17, Cys5-Cys22, Cys12-Cys23 Thr24- C-terminal amidation X=Hydroxyproline

C106H178N34O33S6

0.5 - 200 nM

κ-Conotoxin-RIIIK blocks KV1.2 with high potency.

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

1-2 Business Days

Israel/IL

≥99% (HPLC)

740843-55-4

Lyophilized

Contact Alomone Labs for technical support and product customization

LXSCCSLNLRLCXVXACKRNXCCT-NH2

Yes

12352202

yes

no