µ-Conotoxin SxIIIA | Alomone Labs STC-645 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

µ-Conotoxin SxIIIA

catalog :

STC-645

more info or order :

Alomone Labs product webpage

image

image 1 :

Alomone Labs Serotonin hydrochlorideactivates 5-HT3A receptors expressed in HEK 293T cells. - 5-HT3A receptor currents were elicited by 1 or 10 M Serotonin hydrochloride (#S-165) as indicated. 0.1 M failed to activate currents here.

image 2 :

Alomone Labs -Conotoxin SxIIIA inhibits NaV1.4 currents in Xenopus oocytes. - A. Time course of-Conotoxin SxIIIA(#STC-645) blocking action on maximum NaV1.4 current amplitude. Maximum peak current amplitudes were plotted as a function of time. Membrane potential was held at -100 mV and oocytes were stimulated by a 100 ms voltage step to 0 mV. 50 nM -Conotoxin SxIIIA was perfused as indicated by the bar (green) for 200 sec. B. Superimposed examples of NaV1.4 channel peak current in the absence (control) and presence (green) of 50 nM -Conotoxin SxIIIA (taken from the experiment in A).

product information

cat :

STC-645

SKU :

STC-645_0.1 mg

Product Name :

µ-Conotoxin SxIIIA

Group Type :

Non Antibodies

Product Type :

Proteins

Accession :

P0C8V2

Accession Number :

https://www.uniprot.org/uniprotkb/P0C8V2/entry

Applications :

Electrophysiology

Formulation :

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Reconstitution and Solubility :

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Storage Before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Origin :

Conus striolatus (Cone snail)

Source :

Synthetic peptide

Gene ID :

SCN4A, SCN1A, SCN2A, SCN8A

Product Page - Scientific background :

Voltage-gated sodium (NaV) channels contribute to physiological and pathophysiological electrical signaling in nerve and muscle cells. They play a role in the generation and propagation of action-potential in excitable cells. Eukaryotic NaV channels are heteromeric membrane proteins composed of a pore-forming α-subunit and auxiliary β-subunits1.µ-Conotoxin SxIIIA is a three-disulfide bridged peptide conotoxin originally isolated from the venom of the Conus striolatus cone snail. SxIIIA is a blocker of the voltage-gated NaV1.4 channel and has an effective concentration of 50-100 nM and an IC50 of 7 nM for the channel. µ-Conotoxin SxIIIA and other toxins derived from venomous animals such as spiders, scorpions, mollusks or plants exhibit promising therapeutic and specifically analgesic properties.

Supplier :

Alomone Labs

Target :

NaV1.4 Na+ channels

Long Description :

A Blocker of NaV1.4 Channels

Short Description :

A Blocker of NaV1.4 Channels

MW :

2271.7 Da

Synonyms :

Mu-conotoxin SxIIIA

Modifications :

Disulfide bonds between: Cys2-Cys15, Cys3-Cys20 and Cys10-Cys21

Molecular formula :

C86H151N33O27S6

Effective Concentration :

50 - 100 nM

Activity :

µ-Conotoxin SxIIIA is a NaV1.4 channel blocker1.

Storage of solutions :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Purity :

≥99% (HPLC)

Form :

Lyophilized

Comment :

Contact Alomone Labs for technical support and product customization

Sequence :

RCCTGKKGSCSGRACKNLKCCA-OH

Is Toxin :

Yes

UNSPSC :

12352202

Bioassay Tested :

yes

Steril endotoxin free :

more info or order :

Alomone Labs product webpage