chemical

µ-Conotoxin CnIIIC

STC-640

STC-640_0.1 mg

µ-Conotoxin CnIIIC

Non Antibodies

Proteins

I1SB07

https://www.uniprot.org/uniprotkb/I1SB07/entry

Electrophysiology

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Conus consors (Singed cone)

Synthetic peptide

CHRNA3,CHRNA4,CHRNA7 ,CHRNB2,SCN2A,SCN4A

µ-Conotoxin CnIIIC, a 22-residue conopeptide from Conus consors belonging to µ-conopeptide family, is a very potent, selective and durable antagonist of NaV channels. This toxin blocks both voltage-gated Na+ channels and neuronal nicotinic acetylcholine receptor (nAChR). µ-Conotoxin CnIIIC inhibits the rat skeletal muscle NaV1.4 with IC50 of 1.3 nM and the rat brain NaV1.2 expressed in HEK-293 cells. A low inhibition is also observed for mouse neuronal NaV1.6 and mouse NaV1.7. µ-Conotoxin CnIIIC modestly blocks nAChR α3/β2 subtype with IC50 values of 450 nM (partially reversible) and, to a lesser extent, α7 and α4/β2 subtypes (reversible) expressed in Xenopus oocytes. in vitro, it decreases twitch tension in mouse hemidiaphragms (IC50 = 150 nM) and displays a high blocking effect in mouse extensor digitorum longus muscles (IC50 = 46 nM).

Alomone Labs

NaV channels, α3/β2 nAChR

A Blocker of NaV Channels and an Antagonist of nAChRs

A Blocker of NaV Channels and an Antagonist of nAChRs

2375.7 Da

Mu-Conotoxin CnIIIC

Disulfide bonds between: Cys3-Cys15, Cys4-Cys21 and Cys10-Cys22 Cys22 - C-terminal amidation Z - Pyrrolidone carboxylic acid (Glp)

C92H139N35O28S6

0.5 - 1 µM

µ-Conotoxin CnIIIC blocks NaV channels and α3/β2 nAChRs.

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

1-2 Business Days

Israel/IL

≥99% (HPLC)

Lyophilized

Contact Alomone Labs for technical support and product customization

ZGCCNGPKGCSSKWCRDHARCC-NH2

Yes

12352202

yes

no