αC-Conotoxin PrXA | Alomone Labs STC-620 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

αC-Conotoxin PrXA

catalog :

STC-620

more info or order :

Alomone Labs product webpage

image

image 1 :

Alomone Labs ?C-Conotoxin PrXA inhibits muscle ?1/?1/?/? nAChR heterologously expressed inXenopusoocytes. - A. Time course of?C-Conotoxin PrXA(#STC-620) action on muscle ?1/?1/?/? nAChR. Current amplitudes were plotted as a function of time. Membrane potential was held at ?80 mV and oocytes were stimulated by exposure to 100 ?M acetylcholine every 100 seconds. 20 nM (for 6 min) ?C-Conotoxin PrXA were perfused during the period marked by the bar as indicated. B. Superimposed traces of muscle nAChR channel current in the absence (black) and presence (green) of 20 nM ?C-Conotoxin PrxA (taken from the experiment in A).

product information

cat :

STC-620

SKU :

STC-620_0.1 mg

Product Name :

αC-Conotoxin PrXA

Group Type :

Non Antibodies

Product Type :

Proteins

Accession :

P0C8S5

Accession Number :

https://www.uniprot.org/uniprotkb/P0C8S5/entry

Applications :

Electrophysiology

Formulation :

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Reconstitution and Solubility :

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Storage Before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Origin :

Conus parius (Cone snail)

Source :

Synthetic peptide

Gene ID :

CHRNA1,CHRNB1,CHRND,CHRNE,CHRNG

Product Page - Scientific background :

αC-Conotoxin PrXA is a synthetic peptide purified to homogeneity, originally isolated from the Conus parius venom1. αC-Conotoxin PrXA reversibly blocks both adult and fetal mammalian muscle α1/β1/ε/δ and α1/β1/γ/δ nicotinic ACh receptors1.

Supplier :

Alomone Labs

Target :

α1/β1/γ/δ nAChR

Long Description :

A Potent Antagonist of Mammalian Muscle nAChR

Short Description :

A Potent Antagonist of Mammalian Muscle nAChR

MW :

3541 Da

Synonyms :

αC-PrXA, Alpha-conotoxin PrXA

Modifications :

Disulfide bond between: Cys13-Cys20 X = Hydroxyproline Glu32 - C-terminal amidation

Molecular formula :

C160H247N43O44S2

Effective Concentration :

1 - 20 nM

Activity :

αC-Conotoxin PrXA reversibly blocks both adult and fetal mammalian muscle α1/β1/ε/δ and α1/β1/γ/δ nicotinic ACh receptor1.

Storage of solutions :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Purity :

≥98% (HPLC)

Form :

Lyophilized

Comment :

Contact Alomone Labs for technical support and product customization

Sequence :

TYGIYDAKPXFSCAGLRGGCVLPXNLRXKFKE-NH2

Is Toxin :

Yes

UNSPSC :

12352202

Bioassay Tested :

yes

Steril endotoxin free :

more info or order :

Alomone Labs product webpage