αA-Conotoxin PIVA | Alomone Labs STC-600 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

αA-Conotoxin PIVA

catalog :

STC-600

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Kiss T, Krajcs N, Pirger Z, HernÃ¡di L. Nicotinic acetylcholine receptors containing the Î±7-like subunit mediate contractions of muscles responsible for space positioning of the snail, Helix pomatia L. tentacle. PLoS ONE. 2014;9:e109538 pubmed publisher

image

image 1 :

Alomone Labs ?A-Conotoxin PIVA inhibits muscle fetal ?1/?1/?/? nAChR heterologously expressed inXenopusoocytes. - Time course of?A-Conotoxin PIVA(#STC-600) action on muscle ?1?1?? nAChR. Current amplitudes were plotted as a function of time. Membrane potential was held at ?80 mV and oocytes were stimulated by exposure to 100 M acetylcholine every 50 seconds. 12.5 nM (for 200 sec) 25 nM (for 300 sec) and 50 nM (for 200 sec) ?A-Conotoxin PIVA were perfused during the period marked by the bar as indicated.

product information

cat :

STC-600

SKU :

STC-600_0.1 mg

Product Name :

αA-Conotoxin PIVA

Group Type :

Non Antibodies

Product Type :

Proteins

Accession :

P55963

Accession Number :

https://www.uniprot.org/uniprotkb/P55963/entry

Applications :

Electrophysiology

Formulation :

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Reconstitution and Solubility :

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Storage Before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Origin :

Conus purpurascens (Purple cone)

Source :

Synthetic peptide

Gene ID :

CHRNA1,CHRNB1,CHRND,CHRNE,CHRNG

Product Page - Scientific background :

αA-Conotoxin PIVA is the first member of a new family of nAChR-targeted Conus peptides, named short αA-conotoxins1. It is a peptide toxin originally isolated from Conus Purpurascens (Purple cone) venom1.This peptide reversibly blocks postsynaptic muscle fetal α1/β1/γ/δ nicotinic ACh receptor1,2 . It also blocks postsynaptic muscle adult α1/β1/ε/δ nicotinic ACh receptor, with lower affinity (IC50 of 22 nM to the adult compared to 2.3 nM to the fetal nAChR)2.αA-conotoxin PIVA has a strikingly different amino acid sequence and a Cys framework from all other nAChR-targeted peptides previously characterized from Conus venoms. Despite the striking structural divergence, the peptide blocks the ACh binding site of the nAChR at the neuromuscular junction, similar to the well known typical of a-conotoxins1.The characterization of the short αA-conotoxins revealed diverse kinetics of a block of the fetal muscle nAChR, particularly in dissociation rates. The structure-function relationships of native αA-conotoxins revealed a single amino acid locus (alternatively either His or Pro) that is a critical determinant of the dissociation kinetics2.

Supplier :

Alomone Labs

Target :

α1/β1/γ/δ nAChR

Long Description :

A Potent Antagonist of Muscle Fetal α1/β1/γ/δ nAChR

Short Description :

A Potent Antagonist of Muscle Fetal α1/β1/γ/δ nAChR

MW :

2649 Da

Synonyms :

Alpha-A-conotoxin PIVA, Alpha-conotoxin PIVA

Modifications :

Disulfide bonds between: Cys2-Cys16, Cys3-Cys11 and Cys14-Cys23 X = Hydroxyproline Gln25 - C-terminal amidation

Molecular formula :

C103H150N34O37S6

Effective Concentration :

1 - 20 nM

Activity :

αA-Conotoxin PIVA reversibly blocks muscle α1/β1/γ/δ nicotinic ACh receptor1,2.

Storage of solutions :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Purity :

≥98% (HPLC)

Form :

Lyophilized

Comment :

Contact Alomone Labs for technical support and product customization

Sequence :

GCCGSYXNAACHXCSCKDRXSYCGQ-NH2

Is Toxin :

Yes

UNSPSC :

12352202

Bioassay Tested :

yes

Steril endotoxin free :

more info or order :

Alomone Labs product webpage