α-Conotoxin LtIA | Alomone Labs STC-550 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

α-Conotoxin LtIA

catalog :

STC-550

more info or order :

Alomone Labs product webpage

image

image 1 :

Alomone LabsPalonosetron hydrochlorideblocks 5-HT3A receptors expressed in HEK 293T cells. - 5-HT3A receptor currents were elicited with 10 M 5-HT delivered every 3 minutes.Palonosetron hydrochloride(#P-225) was applied 30 seconds before stimulation at 0.5 1 and 20 nM as indicated and completely inhibited the 5-HT induced current in a dose dependent and reversible manner.

product information

cat :

STC-550

SKU :

STC-550_0.5 mg

Product Name :

α-Conotoxin LtIA

Group Type :

Non Antibodies

Product Type :

Proteins

Accession :

Q2I2R8

Accession Number :

https://www.uniprot.org/uniprotkb/Q2I2R8/entry

Applications :

Electrophysiology

Formulation :

Lyophilized from double distilled water (ddH2O).

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Reconstitution and Solubility :

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Storage Before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Origin :

Conus litteratus (Lettered cone)

Source :

Synthetic peptide

Gene ID :

CHRNA3,CHRNB2, CHRNA6, CHRNB3

Product Page - Scientific background :

α-Conotoxin LtIA is a 16 amino acid peptidyl toxin isolated from the Conus litteratus (Lettered cone) venom1. It belongs to the α-Conotoxin superfamily, although it lacks the highly conserved Ser-Xaa-Pro sequence. It reversibly blocks α3/β2 nicotinic ACh channel receptor with an IC50 of 9.8 nM1.Little or no block was obtained by 10 fold concentrations of the toxin on α4/β2 and α3/ÃŸ4 nicotinic ACh receptors1.α-Conotoxin LtIA was substantially less potent on α6/α3/β4 versus α6/α3/β2/β3 nAChRs. In contrast, there was little or no block by α-Conotoxin LtIA at 10 µM on other nAChR, including α1/β1/δ/ε, α2/β2, α2/β4, α4/β2, α4/β4, α7, and α9/α101.

Supplier :

Alomone Labs

Target :

α3/β2 nAChR

Long Description :

A Novel Antagonist of α3/β2 nAChR

Short Description :

A Novel Antagonist of α3/β2 nAChR

MW :

1601.9 Da

Synonyms :

Alpha-conotoxin Lt1a

Modifications :

Disulfide bonds between: Cys2-Cys8 and Cys3-Cys16

Molecular formula :

C62H100N22O20S4

Effective Concentration :

1 - 10 µM

Activity :

α-Conotoxin LtIA reversibly blocks α3/β2 nicotinic ACh channel receptor1. Little or no block was obtained by 10 fold concentrations of the toxin on α4/β2 and α3/β4 nicotinic ACh receptors.1

Storage of solutions :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Purity :

≥98% (HPLC)

Form :

Lyophilized

Comment :

Contact Alomone Labs for technical support and product customization

Sequence :

GCCARAACAGIHQELC-OH

Is Toxin :

Yes

UNSPSC :

12352202

Bioassay Tested :

yes

Steril endotoxin free :

more info or order :

Alomone Labs product webpage