chemical

µ-Conotoxin BuIIIA

STC-540

STC-540_0.1 mg

µ-Conotoxin BuIIIA

Non Antibodies

Proteins

C1J5M5

https://www.uniprot.org/uniprotkb/C1J5M5/entry

Electrophysiology

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Conus bullatus (Bubble cone)

Synthetic peptide

SCN2A,SCN4A

μ-Conotoxins (μ-CTX) are peptide toxins that block voltage-gated Na+ channels (VGSCs), which are responsible for the influx of Na+ ions during action potentials in excitable tissues1.µ-Conotoxin BuIIIA is originally isolated from the venom of the cone snail Conus bullatus and acts as a selective and potent blocker of voltage-gated NaV1.2 and NaV1.4 channels. The conotoxin inhibits Na+ flux by blocking the Na+ channel pore.BuIIIA is considered to be part of a novel class of μ-conopeptides discovered from a newly defined class of fish-hunting cone snails, the Textilia clade. In addition, Conus bullatus peptides help define a new branch of the M-superfamily of conotoxins, namely M-5 due to a unique N-terminal extension. This N-terminus also exists in µ-Conotoxin BuIIIB and BuIIIC but absent in all other μ-conotoxins2,3.µ-Conotoxin BuIIIA is used to determine the structure-activity relationship of voltage-gated Na+ channels3.

Alomone Labs

NaV1.2, NaV1.4 channels

A Selective and Potent Blocker of NaV1.2 and NaV1.4 Channels

A Selective and Potent Blocker of NaV1.2 and NaV1.4 Channels

2751 Da

Mu-conotoxin BuIIIA

Disulfide bonds between: Cys5-Cys16, Cys6-Cys22, and Cys12-Cys23 Cys23 - C-terminal amidation

C106H172N44O31S6

100 nM - 1 µM

µ-Conotoxin BuIIIA is a potent blocker of voltage-gated NaV1.2 and NaV1.4 channels.1

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

1-2 Business Days

Israel/IL

≥98% (HPLC)

Lyophilized

Contact Alomone Labs for technical support and product customization

VTDRCCKGKRECGRWCRDHSRCC-NH2

Yes

12352202

yes

no