αA-Conotoxin OIVA | Alomone Labs STC-520 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

αA-Conotoxin OIVA

catalog :

STC-520

more info or order :

Alomone Labs product webpage

image

image 1 :

Alomone LabsPalonosetronblocks 5-HT3A receptors expressed in HEK 293T cells. - 5-HT3A receptor currents were elicited with 10 M 5-HT delivered every 3 minutes.Palonosetron(#P-226) was applied 30 seconds before stimulation at 0.5 1 and 20 nM as indicated and completely inhibited the 5-HT induced current in a dose dependent and reversible manner.

image 2 :

Alomone Labs ?A-Conotoxin-OIVA inhibits the muscle (?1/?1/?/?) nAChR channels heterologously expressed inXenopusoocytes. - A. Time course of?A-Conotoxin OIVA(#STC-520) action on ?1/?1/?/? nAChR channels. Current amplitudes were plotted as a function of time. Membrane potential was held at ?80 mV and oocytes were stimulated by exposure to solution containing 100 M ACh + 3 M PNU-120596 every 50 seconds. Muscle nAChR currents are inhibited by 2.5 min application of 100 nM (green) and 250 nM (red) (as indicated). B. Superimposed traces of ?1/?1/?/? nAChR channel current in the absence (control) and presence of 100 nM (green) and 250 nM ?A-Conotoxin OIVA(red) (taken from the experiment in A).

product information

cat :

STC-520

SKU :

STC-520_0.1 mg

Product Name :

αA-Conotoxin OIVA

Group Type :

Non Antibodies

Product Type :

Proteins

Accession :

P69746

Accession Number :

https://www.uniprot.org/uniprotkb/P69746/entry

Applications :

Electrophysiology

Formulation :

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Reconstitution and Solubility :

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Storage Before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Origin :

Conus obscurus (Obscure cone) (Conus halitropus)

Source :

Synthetic peptide

Gene ID :

CHRNA1,CHRNB1,CHRND,CHRNG

Product Page - Scientific background :

αA-Conotoxin OIVA (αA-OIVA) is a peptidyl toxin originally isolated from Conus obscures and belongs to the family of short αA-conotoxins (αAS) which includes αA-OIVA, αA-OIVB, αA-PeIVA, and αA-PeIVB. αA-Conotoxin OIVA blocks the fetal muscular subtype nicotinic acetylcholine receptor (α1/β1/γ/δ subunits) (IC50 = 0.51 nM) with 600-fold greater affinity than the adult subtype (IC50 = 310 nM). It completely blocks the elicited currents and it dissociates very slowly from the fetal muscle receptor. This toxin causes an apparently irreversible paralysis upon intramuscular injection into gold fish.1

Supplier :

Alomone Labs

Target :

α1/β1/γ/δ nAChR

Long Description :

An Antagonist of α1/β1/γ/δ (fetal muscle) nAChR

Short Description :

An Antagonist of α1/β1/γ/δ (fetal muscle) nAChR

MW :

1848 Da

Synonyms :

Alpha-A-O4a, Alpha-A-OIVA

Modifications :

Disulfide bonds between: Cys1-Cys9, Cys2-Cys14 and Cys12-Cys18 X=Hydroxyproline Cys18 - C-terminal amidation

Molecular formula :

C70H110N24O23S6

Effective Concentration :

100 - 250 nM

Activity :

αA-Conotoxin OIVA blocks α1/β1/g/d (fetal muscle) nAChR1.

Storage of solutions :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Purity :

≥99% (HPLC)

Form :

Lyophilized

Comment :

Contact Alomone Labs for technical support and product customization

Sequence :

CCGVXNAACHXCVCKNTC-NH2

Is Toxin :

Yes

UNSPSC :

12352202

Bioassay Tested :

yes

Steril endotoxin free :

more info or order :

Alomone Labs product webpage