α-Conotoxin GI | Alomone Labs STC-500 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

α-Conotoxin GI

catalog :

STC-500

more info or order :

Alomone Labs product webpage

image

image 1 :

Alomone Labs ?-Conotoxin GI inhibits the muscle (?1/?1/?/?) nAChR channels heterologously expressed inXenopusoocytes. - A. Time course of?-Conotoxin GI(#STC-500) action on ?1/?1/?/? nAChR channels. Current amplitudes were plotted as a function of time. Membrane potential was held at ?60 mV and oocytes were stimulated by exposure to 1 mM acetylcholine every 100 seconds. 100 nM ?-Conotoxin GI were perfused during the period marked by the bar. B. Superimposed traces of ?1/?1/?/? nAChR channel current in the absence and presence of 100 nM ?-Conotoxin GI (taken from the experiment in A).

product information

cat :

STC-500

SKU :

STC-500_0.5 mg

Product Name :

α-Conotoxin GI

Group Type :

Non Antibodies

Product Type :

Proteins

Accession :

X5I9Y2

Accession Number :

https://www.uniprot.org/uniprotkb/X5I9Y2/entry

Applications :

Electrophysiology

Formulation :

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Reconstitution and Solubility :

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Storage Before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Origin :

Conus geographus (Geography cone) (Nubecula geographus)

Source :

Synthetic peptide

Gene ID :

CHRNA1,CHRNB1,CHRND,CHRNG, CHRNE

Product Page - Scientific background :

α-Conotoxin GI is a 13 amino acid peptidyl toxin isolated from the Conus geographus (Geography cone) venom1,2,3. It belongs to the Conotoxin A superfamily and reversibly blocks α/δ nicotinic ACh channel receptors (nAChR).α-Conotoxin GI reversibly inhibits the high affinity α/δ site on mouse muscle-derived BC3H-1 receptor, a nAChR expressed on postsynaptic membranes4,5. Other low site (α/γ site) on nicotinic receptors from Torpedo californica electric organ is also inhibited by α-Conotoxin GI5.

Supplier :

Alomone Labs

Target :

α1/β1/δ/γ nAChR

Long Description :

A Potent Antagonist of α1/β1/δ/γ nAChR

Short Description :

A Potent Antagonist of α1/β1/δ/γ nAChR

MW :

1437.6 Da

Synonyms :

α-Conotoxin G1, Alpha-conotoxin GI

Modifications :

Disulfide bonds between: Cys2-Cys7, Cys3-Cys13 Cys13 - C-terminal amidation

Molecular formula :

C55H80N20O18S4

Effective Concentration :

10 nM - 1 μM

Activity :

α-Conotoxin GI inhibits α/δ site on mouse muscle-derived BC3H-1 nicotinic acetylcholine receptors (nAChR) expressed on postsynaptic membranes. Other site (α/γ site) on nicotinic receptors from Torpedo californica electric organ is also inhibited by α-Conotoxin GI1,2.

Storage of solutions :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Purity :

≥98% (HPLC)

CAS No :

76862-65-2

Form :

Lyophilized

Comment :

Contact Alomone Labs for technical support and product customization

Sequence :

ECCNPACGRHYSC-NH2

Is Toxin :

Yes

UNSPSC :

12352202

Bioassay Tested :

yes

Steril endotoxin free :

more info or order :

Alomone Labs product webpage