ω-Conotoxin SO3 | Alomone Labs STC-470 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

ω-Conotoxin SO3

catalog :

STC-470

more info or order :

Alomone Labs product webpage

image

image 1 :

Alomone Labs ?-Conotoxin SO3inhibits CaV2.2 channels heterologously expressed inXenopusoocytes. - A. Time course of?-Conotoxin SO3(#STC-470) blocking action on CaV2.2 currents (?1B + ?2?1+ ?1). Maximum current amplitude was plotted as a function of time. Membrane potential was held at -100 mV and cells were stimulated in the presence of 2 mM Ba2+by a 100 ms voltage ramp to +60 mV. 50 nM ?-Conotoxin SO3were perfused as indicated by the bar (green) during 150 s. B. Superimposed examples of CaV2.2 channel current in the absence (control) and presence (green) of 50 nM ?-Conotoxin SO3(taken from the experiment in A).

product information

cat :

STC-470

SKU :

STC-470_0.1 mg

Product Name :

ω-Conotoxin SO3

Group Type :

Non Antibodies

Product Type :

Proteins

Accession :

Q9XZK2

Accession Number :

https://www.uniprot.org/uniprotkb/Q9XZK2/entry

Applications :

Electrophysiology

Formulation :

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Reconstitution and Solubility :

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Storage Before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Origin :

Conus striatus (Striated cone)

Source :

Synthetic peptide

Gene ID :

CACNA1B

Product Page - Scientific background :

ω-conotoxin SO-3 is a peptide toxin originally isolated from the venom of Conus striatus. It is a selective blocker of N-type CaV channels. SO-3 inhibits INa and IK with IC50 values of 0.49 and 1.6 mM respectively. ω-conotoxin SO-3 does not show any inhibiting effects on L-type, P/Q-type and R-type currents at 3 µM concentration and has no effect on NaV currents, delayed rectifier K+ currents and transient outward K+ currents. ω-conotoxin SO-3 displays an analgesic potency similar to ω-conotoxin MVIIA in a range of acute and chronic pain models in rodents, but has less adverse effects compared with identical dosages of ω-conotoxin MVIIA injected intrathecally1.

Supplier :

Alomone Labs

Target :

CaV2.2 Ca2+ channels

Long Description :

A Blocker of N-Type Ca2+ Channels

Short Description :

A Blocker of N-Type Ca2+ Channels

MW :

2561 Da

Synonyms :

SO3

Modifications :

Disulfide bonds between: Cys1-Cys16, Cys8-Cys20, and Cys15-Cys25 Cys25 - C-terminal amidation

Molecular formula :

C100H166N36O31S6

Effective Concentration :

20 - 500 nM

Activity :

ω-Conotoxin SO3 is a selective N-type Ca2+ channel inhibitor1.

Storage of solutions :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Purity :

≥98% (HPLC)

CAS No :

441284-32-8

Form :

Lyophilized

Comment :

Contact Alomone Labs for technical support and product customization

Sequence :

CKAAGKPCSRIAYNCCTGSCRSGKC-NH2

Is Toxin :

Yes

UNSPSC :

12352202

Bioassay Tested :

yes

Steril endotoxin free :

more info or order :

Alomone Labs product webpage