µ-Conotoxin SIIIA | Alomone Labs STC-450 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

µ-Conotoxin SIIIA

catalog :

STC-450

more info or order :

Alomone Labs product webpage

image

image 1 :

Alomone Labs -Conotoxin SIIIA inhibits NaV1.2 channels heterologously expressed inXenopusoocytes. - A. Time course of channel activity and inhibition in the presence of 1 M -Conotoxin SIIIA(#STC-450) applied for 10 min (indicated as bar). Currents were elicited by application of voltage ramp from a holding potential of -80 mV to 0 mV in 100 msec delivered every 10 seconds. B. Example of superimposed current traces before (black) and during (green) 1 M application of -Conotoxin SIIIA.

product information

cat :

STC-450

SKU :

STC-450_0.1 mg

Product Name :

µ-Conotoxin SIIIA

Group Type :

Non Antibodies

Product Type :

Proteins

Accession :

Q86DU6

Accession Number :

https://www.uniprot.org/uniprotkb/Q86DU6/entry

Applications :

Electrophysiology

Formulation :

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Reconstitution and Solubility :

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Storage Before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Origin :

Conus striatus (Striated cone)

Source :

Synthetic peptide

Gene ID :

SCN1A,SCN2A,SCN3A,SCN4A,SCN8A

Product Page - Scientific background :

µ-Conotoxin SIIIA is a synthetic peptide purified to homogeneity, originally isolated from the Conus striatus venom1. µ-Conotoxin SIIIA reversibly blocks TTX-sensitive voltage-gated Na+ channels in small-diameter DRG neurons.1,2 It also irreversibly blocks NaV1.2, NaV1.3 and NaV1.4 channels expressed in Xenopus oocytes in a dose- and time-dependent manner3. No inhibition is detected on NaV1.5, NaV1.7 or NaV1.8 channels4,5.

Supplier :

Alomone Labs

Target :

NaV1.2, NaV1.3, NaV1.4 Na+ channels

Long Description :

A Blocker of TTX-Sensitive NaV1.2, NaV1.3 and NaV1.4 Voltage-Gated Na+ Channels

Short Description :

A Blocker of TTX-Sensitive NaV1.2, NaV1.3 and NaV1.4 Voltage-Gated Na+ Channels

MW :

2207.5 Da

Synonyms :

Mu-conotoxin SIIIA

Modifications :

Disulfide bonds between: Cys3-Cys13, Cys4-Cys19, Cys8-Cys20 Z1 - Pyrrolidone carboxylic acid (Glp) Cys20 - C-terminal amidation

Molecular formula :

C83H123N33O27S6

Effective Concentration :

0.5 - 3 µM

Activity :

µ-Conotoxin SIIIA blocks TTX-sensitive voltage-gated Na+ channels in small-diameter DRG neurons1,2. It also irreversibly blocks NaV1.2, NaV1.3 and NaV1.4 channels expressed in Xenopus oocytes in a dose- and time-dependent manner3. No inhibition is detected on NaV1.5, NaV1.7 or NaV1.8 channels4,5.

Storage of solutions :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Purity :

≥98% (HPLC)

Form :

Lyophilized

Comment :

Contact Alomone Labs for technical support and product customization

Sequence :

ZNCCNGGCSSKWCRDHARCC-NH2

Is Toxin :

Yes

UNSPSC :

12352202

Bioassay Tested :

yes

Steril endotoxin free :

more info or order :

Alomone Labs product webpage