ω-Conotoxin CVIC | Alomone Labs STC-420 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

ω-Conotoxin CVIC

catalog :

STC-420

more info or order :

Alomone Labs product webpage

image

image 1 :

Peptide (C)EFKDSNSSLHRGPS corresponding to amino acid residues 53-66 of rat VMAT1 (AccessionQ01818). 1stluminal loop.

image 2 :

Alomone Labs ?-Conotoxin CVIC inhibits CaV2.2 channels expressed in Xenopus oocytes. - A. Time course of ?-Conotoxin CVIC (#STC-420) action on CaV2.2 maximum current amplitude. Current amplitudes were plotted as a function of time. Membrane potential was held at -100 mV and oocytes were stimulated by a 100 ms voltage ramp to +50 mV in 2 mM Ba2+. 50 nM ?-Conotoxin CVIC were perfused as indicated by the bar (green) for 5 min. B. Superimposed examples of CaV2.2 channel maximum peak current in the absence (control) and presence (green) of 50 nM ?-Conotoxin CVIC (taken from the experiment in A).

product information

cat :

STC-420

SKU :

STC-420_0.1 mg

Product Name :

ω-Conotoxin CVIC

Group Type :

Non Antibodies

Product Type :

Proteins

Accession :

P58919

Accession Number :

https://www.uniprot.org/uniprotkb/P58919/entry

Applications :

Electrophysiology

Formulation :

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Reconstitution and Solubility :

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Storage Before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Origin :

Conus catus (Cat cone)

Source :

Synthetic peptide

Gene ID :

CACNA1A,CACNA1B

Product Page - Scientific background :

ω-Conotoxin CVIC is a peptide toxin originally isolated from the cat cone (Conus Catus). It is a blocker of voltage-gated N-,P- and Q-type CaV channels. CVIC has an effective concentration of 10-50 nM with an IC50 of 7.6 nM for N-type channels and 31 nM for P- and Q-type channels. Compared to other ω-conotoxins, CVIC is a fairly poor inhibitor of N-type channels and only a moderate inhibitor of P- and Q-type channels. Cloning and sequencing of mRNA transcripts from the venom ducts of the sea-snail failed to isolate CVIC's mRNA transcript thus suggesting that this ω-conotoxin is not found abundantly in the Conus Catus species and may be found in other species of the cone snail family1.

Supplier :

Alomone Labs

Target :

N-, P/Q-type channels

Long Description :

A Blocker of N-, P/Q-Type Ca2+ Channels

Short Description :

A Blocker of N-, P/Q-Type Ca2+ Channels

MW :

2783 Da

Synonyms :

Omega-conotoxin CVIC

Modifications :

Disulfide bonds between: Cys1-Cys16, Cys8-Cys20, and Cys15-Cys26 Cys26 - C-terminal amidation

Molecular formula :

C107H180N38O35S7

Effective Concentration :

10 - 50 nM

Activity :

ω-Conotoxin CVIC is a blocker of N-, P/Q-type CaV channels1.

Storage of solutions :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Purity :

≥98% (HPLC)

Form :

Lyophilized

Comment :

Contact Alomone Labs for technical support and product customization

Sequence :

CKGKGQSCSKLMYDCCTGSCSRRGKCS-NH2

Is Toxin :

Yes

UNSPSC :

12352202

Bioassay Tested :

yes

Steril endotoxin free :

more info or order :

Alomone Labs product webpage