µ-Conotoxin PIIIA | Alomone Labs STC-400 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

µ-Conotoxin PIIIA

catalog :

STC-400

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Feng B, Zhu Y, La J, Wills Z, Gebhart G. Experimental and computational evidence for an essential role of NaV1.6 in spike initiation at stretch-sensitive colorectal afferent endings. J Neurophysiol. 2015;113:2618-34 pubmed publisher
Kirchhof P, Tal T, Fabritz L, Klimas J, Nesher N, Schulte J, et al. First report on an inotropic peptide activating tetrodotoxin-sensitive, "neuronal" sodium currents in the heart. Circ Heart Fail. 2015;8:79-88 pubmed publisher

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product information

cat :

STC-400

SKU :

STC-400_0.1 mg

Product Name :

µ-Conotoxin PIIIA

Group Type :

Non Antibodies

Product Type :

Proteins

Accession :

P58925

Accession Number :

https://www.uniprot.org/uniprotkb/P58925/entry

Applications :

Electrophysiology

Formulation :

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Reconstitution and Solubility :

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Storage Before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Origin :

Conus purpurascens (Purple cone)

Source :

Synthetic peptide

Gene ID :

SCN1A,SCN2A,SCN3A,SCN4A,SCN8A,SCN9A

Product Page - Scientific background :

µ-Conotoxin PIIIA is a 22 amino acid peptidyl toxin isolated from the Conus purpurascens (Purple cone) venom and belongs to the conotoxin M superfamily1. It reversibly blocks mammalian skeletal muscle voltage-gated Na+ channels (NaV1.4, IC50 of 44 nM), Type II (NaV1.2) and PN1 (NaV1.7) neuronal voltage-gated Na+ channels (IC50 of 640 nM) by binding to site I domain of the channel1-3. In addition, it irreversibly blocks amphibian muscle Na+ channels1.

Supplier :

Alomone Labs

Target :

Various NaV channels

Long Description :

A Potent Blocker of NaV1.2, NaV1.4 and NaV1.7 Na+ Channels

Short Description :

A Potent Blocker of NaV1.2, NaV1.4 and NaV1.7 Na+ Channels

MW :

2604 Da

Synonyms :

μ-Conotoxin P3.7

Modifications :

Disulfide bonds between: Cys4-Cys16, Cys5-Cys21, and Cys11-Cys22 Cys22 - C-terminal amidation. Pro8 , Pro18 - Hydroxyprolines Q1 - Pyrrolidone carboxylic acid (Glp)

Molecular formula :

C103H166N40O28S6

Effective Concentration :

10 nM - 1 μM

Activity :

μ-Conotoxin PIIIA reversibly blocks mammalian skeletal muscle voltage-gated Na+ channels (NaV1.4, IC50 of 44 nM), Type II (NaV1.2) and PN1 (NaV1.7) neuronal voltage-gated Na+ channels (IC50 of 640 nM) by binding to site I domain of the channel. In addition, it blocks irreversibly amphibian muscle Na+ channels1.

Storage of solutions :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Purity :

≥98% (HPLC)

Form :

Lyophilized

Comment :

Contact Alomone Labs for technical support and product customization

Sequence :

QRLCCGFPKSCRSRQCKPHRCC-NH2

Is Toxin :

Yes

UNSPSC :

12352202

Bioassay Tested :

yes

Steril endotoxin free :

more info or order :

Alomone Labs product webpage