α-Conotoxin MI | Alomone Labs STC-370 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

α-Conotoxin MI

catalog :

STC-370

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Nayak T, Vij R, Bruhova I, Shandilya J, Auerbach A. Efficiency measures the conversion of agonist binding energy into receptor conformational change. J Gen Physiol. 2019;151:465-477 pubmed publisher

image

image 1 :

Alomone Labs ?-Conotoxin MI inhibits muscle fetal?1/?1/?/? nAChR heterologously expressed inXenopusoocytes. - Time course of?-Conotoxin MI(#STC-370) action on muscle ?1/?1/?/? nAChR. Current amplitudes were plotted as a function of time. Membrane potential was held at ?80 mV and oocytes were stimulated by exposure to 10 ?M acetylcholine every 100 seconds. 2 nM (for 3 min) and 20 nM (for 3 min) ?-Conotoxin MI were perfused during the period marked by the bar as indicated.

product information

cat :

STC-370

SKU :

STC-370_0.1 mg

Product Name :

α-Conotoxin MI

Group Type :

Non Antibodies

Product Type :

Proteins

Accession :

P01521

Accession Number :

https://www.uniprot.org/uniprotkb/P01521/entry

Applications :

Electrophysiology

Formulation :

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Reconstitution and Solubility :

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Storage Before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Origin :

Conus magus (Magical cone)

Source :

Synthetic peptide

Gene ID :

CHRNA1,CHRNB1,CHRND,CHRNG

Product Page - Scientific background :

α-Conotoxin MI is a 14 amino acid peptidyl toxin isolated from the Conus magus (Magus cone, Magician's cone snail venom)1. It acts on postsynaptic membranes on muscles and blocks mammalian nicotinic ACh channel receptor composed of α1/δ subunits with high potency and α1/γ with a low potency2.Structure activity relationship studies were done in order to map the major amino acids in α-Conotoxin MI and the overall results show that primarily hydrophobic interactions stabilize the complex between the toxin and the α-δ interface of the nAChR3. Proline-6 and Tyrosine-12 dramatically reduced toxin potency at the high-affinity α/δ site while having comparatively little effect on low-affinity α/γ binding4.

Supplier :

Alomone Labs

Target :

α1/β1/γ/δ nAChR

Long Description :

A Potent Antagonist of Muscle α1/β1/γ/δ nAChR

Short Description :

A Potent Antagonist of Muscle α1/β1/γ/δ nAChR

MW :

1493.7 Da

Synonyms :

α-MI, CtxMI, M1

Modifications :

Disulfide bonds between: Cys3-Cys8, Cys4-Cys14 Cys14 - C-terminal amidation

Molecular formula :

C58H88N22O17S4

Effective Concentration :

2 - 20 nM

Activity :

α-Conotoxin MI reversibly blocks fetal muscle α1/β1/γ/δ nicotinic ACh receptor1,2,3.

Storage of solutions :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Purity :

≥98% (HPLC)

CAS No :

83481-45-2

Form :

Lyophilized

Comment :

Contact Alomone Labs for technical support and product customization

Sequence :

GRCCHPACGKNYSC-NH2

Is Toxin :

Yes

UNSPSC :

12352202

Bioassay Tested :

yes

Steril endotoxin free :

more info or order :

Alomone Labs product webpage