chemical

α-Conotoxin MrIC

STC-320

STC-320_0.5 mg

α-Conotoxin MrIC

Non Antibodies

Proteins

F5C3U4

https://www.uniprot.org/uniprotkb/F5C3U4/entry

Electrophysiology

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Conus marmoreus (Marble cone)

Synthetic peptide

CHRNA7

α-Conotoxin MrIC is peptide toxin originally isolated from Conus marmoreus. It is a competitive and selective antagonist of α7 nicotinic acetylcholine receptor (nAChRα7). Peptide toxins belonging to α-conotoxin family are relatively short peptides, between 12−19 amino acid residues and demonstrate high affinity towards nAChRs. They are stabilized by two disulfide bonds1,2.In Ca2+ responses assay to α-Conotoxin MrIC in SH-SY5Y cells endogenously expressing α7 nAChR, α-Conotoxin MrIC elicits concentration-dependent increases in intracellular Ca2+ in the presence of the α7 nAChR-specific positive allosteric modulator PNU120596 with an EC50 value of 1.9 µM2. Interestingly, α-Conotoxin MrIC antagonizes nAChRα7 heterologously expressed in Xenopus oocytes1.nAChRs receptors are responsible for mediating the effects of the neurotransmitter acetylcholine (ACh). They play critical physiologic roles in the central and peripheral nervous system where they regulate neurotransmitter release, cell excitability, neuronal integration, and are involved in functions such as sleep and arousal patterns, fatigue, hunger, anxiety, and pain processing3,4.

Alomone Labs

α7 nAChR

An Antagonist and Partial Agonist of α7 nAChR

An Antagonist and Partial Agonist of α7 nAChR

1835 Da

Mr1.7c

Disulfide bonds between: Cys3-Cys9 and Cys4-Cys17 Cys17 - C-terminal amidation

C74H111N23O24S4

1 - 10 µM

α-Conotoxin MrIC is a selective antagonist of α7 nAChR heterologously expressed in Xenopus oocytes, while acting as an agonist of endogenous α7 nAChR in the prescence of PNU-1205961.

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

1-2 Business Days

Israel/IL

≥98% (HPLC)

Lyophilized

Contact Alomone Labs for technical support and product customization

PECCTHPACHVSNPELC-NH2

Yes

12352202

yes

no