chemical

α-Conotoxin AuIB

STC-310

STC-310_0.1 mg

α-Conotoxin AuIB

Non Antibodies

Proteins

P56640

https://www.uniprot.org/uniprotkb/P56640/entry

Electrophysiology

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Conus aulicus (Princely cone)

Synthetic peptide

CHRNA3,CHRNB4,CHRNA7, GABBR1 ,GABBR2

α-Conotoxin AuIB is a 15 amino acid peptidyl toxin isolated from Conus aulicus1. This toxin inhibits mammalian neuronal α3/β4 nicotinic acetylcholine receptor (nAChR) channels expressed in Xenopus oocytes with an IC50 of 0.75 µM Furthermore, α-Conotoxin AuIB blocks the α3/β4 receptor with >100-fold higher potency than other receptor subunit combinations1.In accordance, α-Conotoxin AuIB (1-5 µM) blocks 20-35% of the nicotine-stimulated norepinephrine release from rat hippocampal synaptosomes1 and the nicotinic ACh receptors on dissociated neurons of the rat parasympathetic ganglia with IC50 of 1.2 nM2.α-Conotoxin AuIB also mediates inhibition of N-type calcium channels at nanomolar concentrations (IC50 = 1.5 nM in rat DRG neurons) via agonism of γ-aminobutyric acid (GABA) G protein-coupled (GABAB) receptors3. GABAB receptors are promising targets for the treatment of various neurological and psychiatric disorders, including pain, depression, and drug addiction. GABAB receptors are widely expressed and distributed in pain-processing pathways at all levels of the neuraxis and play an extensive role in editing and modulating nociceptive inputs. α-Conotoxin AuIB produced reversal of allodynia in the partial nerve ligation (PNL) model3,4.

Alomone Labs

α3/β4 nAChR, GABA(B) receptor agonist

An Antagonist of Mammalian α3/β4 Neuronal nAChR and GABA(B) receptor agonist

An Antagonist of Mammalian α3/β4 Neuronal nAChR and GABA(B) receptor agonist

1572.8 Da

Alpha-conotoxin AuIB

Disulfide bonds between: Cys2-Cys8 and Cys3-Cys15 Cys15 - C-terminal amidation

C65H89N17O21S4

0.1 - 5 μM

α-Conotoxin AuIB potently and specifically blocks mammalian neuronal nAChR channels α3/β41 and modulates high-voltage-activated (HVA) calcium channel through agonism of G-protein-coupled GABAB receptors 2.

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

1-2 Business Days

Israel/IL

≥99% (HPLC)

216299-21-7

Lyophilized

Contact Alomone Labs for technical support and product customization

GCCSYPPCFATNPDC-NH2

Yes

12352202

yes

no