chemical

µ-Conotoxin GIIIA

STC-280

STC-280_0.1 mg

µ-Conotoxin GIIIA

Non Antibodies

Proteins

P01523

https://www.uniprot.org/uniprotkb/P01523/entry

Electrophysiology

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Conus geographus (Geography cone) (Nubecula geographus)

Synthetic peptide

SCN4A, SCN1A, SCN8A

μ-Conotoxin GIIIA is a peptide toxin originally isolated from the venom of Conus geographus. It specifically blocks skeletal muscle Na+ channels (NaV1.4) with an IC50 of 1500 nM1.NaV channels are targets for many toxins, which bind with high affinity to various sites on the channel protein, µ-Conotoxin selectively binds to the NaV1.4 channel, blocks the pore and disabling its normal function, act as an antagonist for VGSCs1.Voltage-gated NaV channels (VGSCs) play a key role in the electric excitability of cells by regulating the influx of sodium ions. VGSCs are responsible for initiating and propagating action potentials, which are essential for the activity of a wide selection of cells and tissues such as neurons, heart and muscle. In mammals, nine different subtypes (NaV1.1-1.9) have been identified, each with distinct distribution.VGSCs acts as a potential drug targets for related disorders such as cardiac and neuropathic diseases2,3.

Alomone Labs

NaV1.4 Na+ channel

A Blocker of NaV1.4 Na+ Channel

A Blocker of NaV1.4 Na+ Channel

2609 Da

G3.9, Geographutoxin I, GTx-I, Myotoxin I

Disulfide bonds between: Cys3-Cys15, Cys4-Cys20 and Cys10-Cys21 X = Hydroxyproline Ala22 - C-terminal amidation

C100H170N38O32S6

50 - 500 nM

µ-Conotoxin GIIIA is a NaV1.4 channel blocker1,2.

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

1-2 Business Days

Israel/IL

≥99% (HPLC)

129129-65-3

Lyophilized

Contact Alomone Labs for technical support and product customization

RDCCTXXKKCKDRQCKXQRCCA-NH2

Yes

12352202

yes

no