chemical

α-Conotoxin EIIB

STC-230

STC-230_0.1 mg

α-Conotoxin EIIB

Non Antibodies

Proteins

C0HKF6

https://www.uniprot.org/uniprotkb/C0HKF6/entry

Electrophysiology

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Conus ermineus (Agate cone) (Chelyconus ermineus)

Synthetic peptide

CHRNA1,CHRNB1,CHRND,CHRNG

α-Conotoxin EIIB is a peptide toxin originally isolated from Conus ermineus (Atlantic fish-hunting cone) venoM It is a potent α1/β1/δ/γ nicotinic acetylcholine receptor antagonist and displays a Ki of 2.2 ± 0.7 nM1. The peptide toxin is stabilized by six hydrogen bonds and disulfides between residues 2 and 7 and 3 and 132.Nicotinic acetylcholine receptors (nAChRs) are ionotropic multisubunit, neurotransmitter-gated receptors of the cholinergic systeM They are assembled from one or more α subunits (α1-α10) alone or together with one or more β subunits (β1-β4)3. Other subunits also co-assemble in the formation of the receptor channel.

Alomone Labs

Muscle nAChR

A Potent Antagonist of α1/β1/δ/γ nAChR

A Potent Antagonist of α1/β1/δ/γ nAChR

1754.5 Da

Alpha-conotoxin EIIB

Disulfide bonds between: Cys5-11, Cys6-16 Cys16 - C-terminal amidation Pro3 - Hydroxyproline Pyrrolidone carboxylic acid (Glp) E1 - Pyrrolidone carboxylic acid (Glp)

C71H103N25O20S4

1 - 50 nM

α-Conotoxin EIIB is a potent α1/β1/δ/γ nAChR antagonist1.

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

1-2 Business Days

Israel/IL

≥99% (HPLC)

Lyophilized

Contact Alomone Labs for technical support and product customization

E*TP*GCCWHPACGKNRC-NH2

Yes

12352202

yes

no