chemical

α-Conotoxin GIC

STC-070

STC-070_0.1 mg

α-Conotoxin GIC

Non Antibodies

Proteins

Q86RB2

https://www.uniprot.org/uniprotkb/Q86RB2/entry

Electrophysiology

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Conus geographus (Geography cone) (Nubecula geographus)

Synthetic peptide

CHRNA6,CHRNA3,CHRNB2,CHRNB4,CHRNA4, CHRNB3

α-conotoxin GIC is a 16 amino acid peptidyl toxin originally isolated from a genomic DNA clone of the marine snail, Conus geographus1. This toxin potently and selectively blocks neuronal α3β2 nicotinic acetylcholine receptors (nAChRs) at very low concentrations (IC50 ~ 1.1 nM)1.α-conotoxin GIC has >100,000-fold selectivity for the neuronal α3β2 subtype versus the muscle subtype1. This toxin belongs to the α4/7-CTx subfamily that primarily targets the vertebrate neuronal nAChRs.Neuronal nAChRs are a heterogeneous family of ligand-gated cation channels that are expressed throughout the brain and involved in a wide range of physiological and pathophysiological processes. These distinct receptor subtypes have a pentameric structure consisting of a homomeric or heteromeric combination of 12 different subunits (α2-α10, β2-β4)2.nAChRs are critically important for neuronal survival and cognitive function, as well as regulation of neurodegenerative diseases, including Alzheimer's and Parkinson's. The nAChR subtypes share a common basic structure, but their biophysical and pharmacological properties depend on their subunit composition. Thus, the subunit makeup of the nAChR subtypes is central to understanding their function in the nervous system and for discovering new subtype-selective drugs2-4.

Alomone Labs

α3β2 nAChRs

A selective and potent α3β2 nAChRs Antagonist

A selective and potent α3β2 nAChRs Antagonist

1609.8 Da

Alpha-conotoxin GIC, α-CTX GIC

Disulfide bonds between: Cys2-Cys8, Cys3-Cys16 Cys16 - C-terminal amidation

C61H92N24O20S4

1 - 20 nM

Reversibly blocks neuronal α3β2 nAChRs.

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

1-2 Business Days

Israel/IL

≥98% (HPLC)

Lyophilized

Contact Alomone Labs for technical support and product customization

GCCSHPACAGNNQHIC-NH2

Yes

12352202

yes

no