chemical

α-Conotoxin MII

STC-040

STC-040_0.1 mg

α-Conotoxin MII

Non Antibodies

Proteins

P56636

https://www.uniprot.org/uniprotkb/P56636/entry

Electrophysiology

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Conus magus (Magical cone)

Synthetic peptide

CHRNA6,CHRNA3,CHRNB2,CHRNB4,CHRNA4,CHRNA7

α-Conotoxin MII is a 16 amino acid peptidyl toxin originally isolated from the venom of the marine snail, Conus magus1. This toxin was initially thought to be a selective antagonist for α3β2 nicotinic acetylcholine receptors (nAChRs)1. Subsequently, it has been shown that α-conotoxin MII is also an α6β2* nAChR subtype selective antagonist2 and it potently blocks β3-containing neuronal nAChRs3.Neuronal nAChRs are a heterogeneous family of ligand-gated cation channels that are expressed throughout the brain and involved in a wide range of physiological and pathophysiological processes. These different receptor subtypes have a pentameric structure consisting of the homomeric or heteromeric combination of 12 different subunits (α2-α10, β2-β4)4.nAChRs are critically important for neuronal survival and cognitive function, as well as regulation of neurodegenerative diseases, including Alzheimer's and Parkinson's. The nAChR subtypes share a common basic structure, but their biophysical and pharmacological properties depend on their subunit composition. Thus, the subunit makeup of the nAChR subtypes is central to understanding their function in the nervous system and for discovering new subtype-selective drugs4-6.

Alomone Labs

α3β2, α6β2 nAChRs

A selective α3β2 and α6β2* subtype nAChRs Antagonist

A selective α3β2 and α6β2* subtype nAChRs Antagonist

1711 Da

Alpha-Ctx MII, Alpha-conotoxin MII, Alpha-MII

Disulfide bonds between: Cys2-Cys8, Cys3-Cys16 Cys16 - C-terminal amidation

C67H103N23O22S4

5 - 20 nM

A potent antagonist of nAChRs composed of α3β2 and α6β2 subunits1,2. Also, potently blocks β3-containing neuronal nicotinic receptors3.

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

1-2 Business Days

Israel/IL

≥98% (HPLC)

175735-93-0

Lyophilized

Contact Alomone Labs for technical support and product customization

GCCSNPVCHLEHSNLC-NH2

Yes

12352202

yes

no