protein

α-Conotoxin BuIA

STC-030

STC-030_0.1 mg

α-Conotoxin BuIA

Non Antibodies

Proteins

P69657

https://www.uniprot.org/uniprotkb/P69657/entry

Electrophysiology

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Conus bullatus (Bubble cone)

Synthetic peptide

CHRNA6,CHRNA3,CHRNB2,CHRNB4,CHRNA4,CHRNA2, CHRNA7

α-Conotoxin BuIA is a 13 amino acid peptidyl toxin that was originally isolated from the fish-eating snail, Conus bullatus1. This toxin exhibits strong antagonistic activity at α6/α3β2β3, α3β2, and α3β4 nicotinic acetylcholine receptors (nAChRs) and has the unique ability to kinetically discriminate between the β2 and β4-containing receptor subtypes, as the off-rates are rapid for β2-subunit, but very slow for β4-containing nAChRs. α-Conotoxin BuIA is a member of the A-superfamily of conotoxins and possess an unusual 4/4 disulfide scaffold. This toxin appears to be a valuable probe to distinguish among nAChRs containing different α and β subunits1-4.nAChRs are pentameric ligand-gated ion channels that are critically important for neuronal survival and cognitive function, as well as regulation of neurodegenerative diseases, including Alzheimer's and Parkinson's. The nAChR subtypes share a common basic structure, but their biophysical and pharmacological properties depend on their subunit composition. Thus, the subunit makeup of the nAChR subtypes is central to understanding their function in the nervous system and for discovering new subtype-selective drugs5-7.

Alomone Labs

α6/α3β2β3, α3β2, and α3β4 nAChRs

A Potent α6/α3β2β3 and α3β2 nAChRs Antagonist

A Potent α6/α3β2β3 and α3β2 nAChRs Antagonist

1311.6 Da

Conotoxin Bu1.3, BuIA, Alpha-conotoxin BuIA

Disulfide bonds between: Cys2-Cys8, Cys3-Cys13 Cys13 - C-terminal amidation

C54H82N14O16S4

10 - 50 nM

A potent α6/α3β2β3, α3β2, and α3β4 nAChRs blocker with a unique ability to kinetically discriminate between the β2 and β4-containing receptor subtypes1.

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

1-2 Business Days

Israel/IL

≥98% (HPLC)

Lyophilized

Contact Alomone Labs for technical support and product customization

GCCSTPPCAVLYC-NH2

Yes

12352202

yes

no