product summary
Loading...
company name :
Alomone Labs
product type :
chemical
product name :
ATX-II
catalog :
STA-700
more info or order :
citations: 19
| Reference |
|---|
image
image 1 :
Alomone Labs ATX-II enhances hNaV1.5 currents in stably transfected HEK cells. - hNaV1.5 currents were elicited by a 50 ms voltage step from the holding potential of -100 mV to -20 mV applied every 20 sec using whole-cell voltage clamp technique (bath solution contains TEA and pipette solution contains CsF). A. Time course showing the effect of 0.05 nM 0.5 nM 5 nM and 50 nMATX-II(#STA-700) on the current area indicating a dose-dependent slowing of the hNaV1.5 inactivation. B. Superimposed traces of hNaV1.5 currents under control conditions and after 4-6 min perfusion with 0.05 nM 0.5 nM 5 nM and 50 nM ATX-II as indicated.
image 2 :
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: - 12.Anti-P2X6 Receptor (extracellular)Antibody (#APR-028) (1:200).34. Anti-P2X6 Receptor (extracellular) Antibody preincubated with the negative control antigen.
product information
cat :
STA-700
SKU :
STA-700_0.1 mg
Product Name :
ATX-II
Group Type :
Non Antibodies
Product Type :
Proteins
Accession :
P01528
Accession Number :
https://www.uniprot.org/uniprotkb/P01528/entry
Applications :
Electrophysiology
Formulation :
Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.
Storage After Reconstitution :
The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.
Reconstitution and Solubility :
Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.
Solubility :
Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.
Storage Before Reconstitution :
The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.
Origin :
Anemonia sulcata (Mediterranean snakelocks sea anemone)
Source :
Synthetic peptide
Gene ID :
SCN1A,SCN2A,SCN3A,SCN4A,SCN5A,SCN8A,SCN9A
Product Page - Scientific background :
ATX-II is a 47 amino acid peptidyl toxin, originally isolated from Anemonia sulcata sea anemone venoM It is a potent neurotoxin, which modulates voltage-gated Na+ channel gating kinetics by delaying its inactivation and prolonging the action potential of excitable membranes.ATX-II has been used as a powerful activator of TTX-sensitive and -insensitive Na+ channels in various excitable tissue and cell types (at concentration range of 10-100 nM).1-9
Supplier :
Alomone Labs
Target :
Various NaV channels
Long Description :
A Modulator of NaV Channels
Short Description :
A Modulator of NaV Channels
MW :
4935 Da
Synonyms :
Neurotoxin 2, Toxin ATX-II, As2, Anemonia viridis toxin 2, Av2, Delta-actitoxin-Avd1c 2, Toxin II
Modifications :
Disulfide bonds between: Cys4-Cys44, Cys6-Cys34 and Cys27-Cys45
Molecular formula :
C213H323N63O61S6
Effective Concentration :
10 - 100 nM
Activity :
ATX-II potently modulates voltage-gated Na+ channel gating kinetics by delaying its inactivation and prolonging the action potential of excitable membranes. ATX-II has been used as a powerful activator of TTX-sensitive and insensitive Na+ channels in various excitable tissue and cell types1-9.
Storage of solutions :
The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.
Lead Time :
1-2 Business Days
Country of origin :
Israel/IL
Purity :
≥98% (HPLC)
CAS No :
60748-45-0
Form :
Lyophilized
Comment :
Contact Alomone Labs for technical support and product customization
Sequence :
GVPCLCDSDGPSVRGNTLSGIIWLAGCPSGWHNCKKHGP
TIGWCCKQ-OH
TIGWCCKQ-OH
Is Toxin :
Yes
UNSPSC :
12352202
Bioassay Tested :
yes
Steril endotoxin free :
no
Cited Application :
Electrophysiology
more info or order :
company information
Alomone Labs
Jerusalem BioPark (JBP), Hadassah Ein Kerem
P.O. Box 4287
Jerusalem 9104201
P.O. Box 4287
Jerusalem 9104201
info@alomone.com
http://www.alomone.com972 2 531 8002
headquarters: Israel
browse more products
questions and comments