Apamin-Biotin | Alomone Labs STA-200-B product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

Apamin-Biotin

catalog :

STA-200-B

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Abiraman K, Tzingounis A, Lykotrafitis G. KCa2 channel localization and regulation in the axon initial segment. FASEB J. 2017;: pubmed publisher
Abiraman K, Sah M, Walikonis R, Lykotrafitis G, Tzingounis A. Tonic PKA Activity Regulates SK Channel Nanoclustering and Somatodendritic Distribution. J Mol Biol. 2016;428:2521-2537 pubmed publisher

image

image 1 :

Alomone Labs Apamin-Biotin blocks rat SK2 channels stably transfected in HEK293T cells. - A. Time course ofApamin-Biotin(#STA-200-B) action on SK2 channels. Current amplitudes were plotted as a function of time. Membrane potential was held at -80 mV and cells were stimulated by a 150 ms voltage ramp from -120 mV to +60 mV every 10 sec. 1 nM 10 nM and 50 nM Apamin-Biotin were perfused during 120 sec as indicated by the bar at 0 mV. B. Superimposed examples of SK2 channel current in the absence (control black) and presence of 1 nM (green) 10 nM (red) and 50 nM (blue) Apamin-Biotin (taken from the experiment in A).

image 2 :

product information

cat :

STA-200-B

SKU :

STA-200-B_10 mcg

Product Name :

Apamin-Biotin

Group Type :

Non Antibodies

Product Type :

Proteins

Applications :

Electrophysiology, Immunofluorescence

Formulation :

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Storage After Reconstitution :

Store the reconstituted solution for the shortest time possible at -20°C. We do not recommend storing the product in working solution for longer than one day. Avoid multiple freeze-thaw cycles.

Reconstitution and Solubility :

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (5 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water to high-micromolar concentrations (50 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Storage Before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Origin :

Apis mellifera (Honeybee)

Source :

Modified synthetic peptide

Gene ID :

KCNN1,KCNN2,KCNN3

Product Page - Scientific background :

Apamin is a natural peptide isolated and purified from Apis mellifera bee venoM Apamin blocks small conductance Ca2+-activated K+ channels (SK). It is specific for the SK1-3 isoforms, but ineffective in blocking other calcium activated K+ channels2. In mammalian cell lines, Apamin blocks expressed hSK1 with an IC50 of ~10 nM3, rSK1 and rSK2 with IC50 of ~3 and

Supplier :

Alomone Labs

Target :

KCa2 K+ channels

Long Description :

A Blocker of Small Conductance Ca2+-Activated K+ Channels (SK Type) Conjugated to Biotin

Short Description :

A Blocker of Small Conductance Ca2+-Activated K+ Channels (SK Type) Conjugated to Biotin

MW :

2367 Da

Synonyms :

APM, Apamine

Modifications :

Disulfide bonds between: Cys1-Cys11, and Cys3-Cys15 His18 - C-terminal amidation LC-Biotin

Effective Concentration :

1 - 50 nM

Activity :

Apamin is a blocker of voltage-independent Ca2+-activated K+ (SK) channels1.

Storage of solutions :

Store the reconstituted solution for the shortest time possible at -20°C. Avoid multiple freeze- thaw cycles. We do not recommend storing the toxin in working solutions for longer than a day.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Form :

Lyophilized

Comment :

Contact Alomone Labs for technical support and product customization

Sequence :

Biotin-CNCKAPETALCARRCQQH-NH2

Is Toxin :

Yes

UNSPSC :

12352202

Bioassay Tested :

yes

Steril endotoxin free :

Cited Application :

Electrophysiology

more info or order :

Alomone Labs product webpage