Azemiopsin | Alomone Labs STA-100 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

Azemiopsin

catalog :

STA-100

more info or order :

Alomone Labs product webpage

image

image 1 :

Alomone LabsAzemiopsin inhibits fetal muscle nAChR (?1/?1/?/?) heterologously expressed inXenopusoocytes. - Time course ofAzemiopsin(#STA-100) action on fetal muscle ?1/?1/?/? nAChR. Current amplitudes were plotted as a function of time. Membrane potential was held at -60 mV and oocytes were stimulated by exposure to 100 M acetylcholine every 200 seconds. 1 M (for 5 min) Azemiopsin was perfused during the period marked by the bar.

product information

cat :

STA-100

SKU :

STA-100_0.1 mg

Product Name :

Azemiopsin

Group Type :

Non Antibodies

Product Type :

Proteins

Accession :

B3EWH2

Accession Number :

https://www.uniprot.org/uniprotkb/B3EWH2/entry

Applications :

Electrophysiology

Formulation :

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Reconstitution and Solubility :

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Storage Before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Origin :

Azemiops feae (Fea's viper)

Source :

Synthetic peptide

Gene ID :

CHRNA1,CHRNB1,CHRND,CHRNE,CHRNG

Product Page - Scientific background :

Azemiopsin is a 21 amino acid peptidyl toxin, isolated from the Azemiops feae viper venom1 and is a synthetic version of the peptide. Unlike similar venom isolates, it does not contain cysteine residues1.Azemiopsin efficiently competes with α-Bungarotoxin (#B-100) for binding to Torpedo nAChR and with lower efficiency to human α7 nAChR. It dose-dependently blocks ACh-induced currents in Xenopus oocytes heterologously expressing human muscle-type nAChR and is more potent against the adult form than the fetal form1. The peptide has no effect on GABA(A) receptors or on 5-HT3 receptors1.Azemiopsin resembles Waglerin-1 (#STW-001) (a disulfide-containing peptide from the Tropidechis wagleri venom) in its biological activity.

Supplier :

Alomone Labs

Target :

α1/β1/γ/δ nAChR

Long Description :

An Antagonist of Muscular α1/β1/γ/δ nAChR

Short Description :

An Antagonist of Muscular α1/β1/γ/δ nAChR

MW :

2541 Da

Molecular formula :

C118H174N38O26

Effective Concentration :

0.5 - 10 µM

Activity :

Azemiopsin blocks the adult form (α1/β1/ε/δ) as well as the fetal form (α1/β1/γ/δ) nAChR1.

Storage of solutions :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Purity :

≥98% (HPLC)

CAS No :

1391936-86-9

Form :

Lyophilized

Comment :

Contact Alomone Labs for technical support and product customization

Sequence :

DNWWPKPPHQGPRPPRPRPKP-OH

Is Toxin :

Yes

UNSPSC :

12352202

Bioassay Tested :

yes

Steril endotoxin free :

more info or order :

Alomone Labs product webpage