chemical

Sarafotoxin S6b

SPE-160

SPE-160_0.1 mg

Sarafotoxin S6b

Non Antibodies

Proteins

P13208

https://www.uniprot.org/uniprotkb/P13208/entry

Aequorin functional assay / Calcium imaging assay

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Atractaspis engaddensis (Israeli burrowing asp) (Israeli mole viper)

Synthetic peptide

EDNRA, EDNRB

Sarafotoxin S6b is a non-selective potent endothelin receptor agonist designed from the Lys-2-Arg-1 dipeptide of the endothelin pro-sequence. Sarafotoxin S6b has the ability to induce contractions of the human isolated coronary artery1,2.Studies have shown that acute infusion of an ET-B receptor agonist into the rat renal medulla leads to natriuresis and diuresis via a mechanism that is NOS1-dependent3. Thus, endothelin receptors pharmacology play an important role in studying the function of the receptors.Endothelin receptors include two subtypes: ET-A and ET-B. They are widely distributed in vascular and nonvascular tissues. ETA receptors are predominantly expressed in peripheral tissues, especially in vascular smooth muscle tissues to mediate vasoconstriction. They are also detected in several different regions of the brain. ET-A receptor has high affinity for endothelin-1 and endothelin-2 and relatively low affinity for endothelin-3, while ET-B receptor has equally high affinity for all endothelin isopeptides4.

Alomone Labs

Endothelin receptors

A Non-Selective and Potent Agonist of Endothelin Receptors

A Non-Selective and Potent Agonist of Endothelin Receptors

2564 Da

SRTX-B, S6B, Sarafotoxin-B

Disulfide bonds between: Cys1-Cys15 and Cys3-Cys11

C110H159N27O34S5

1 pM - 100 nM

Sarafotoxin S6b is an endothelin receptor agonist1,2.

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

1-2 Business Days

Israel/IL

≥98% (HPLC)

116303-65-2

Lyophilized

Contact Alomone Labs for technical support and product customization

CSCKDMTDKECLYFCHQDVIW-OH

Yes

12352202

yes

no