SB 203580 | Alomone Labs S-370 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

SB 203580

catalog :

S-370

more info or order :

Alomone Labs product webpage

image

image 1 :

Alomone Labs SB 203580 inhibits the phosphorylation of Elk1 and Atf2 via p38 in 3T3-L1 cells. - Cells were grown to 70% confluence and serum starved for 3 h. The cells were then incubated for 2 h with various concentrations ofSB 203580(#S-370) and stimulated with 5 MAnisomycin(#A-520). Cell proteins were resolved by SDS-PAGE and probed with anti-phospho-p38 MAPK antip38 MAPK anti-phospho-Elk1 and anti-phospho-Atf2 as indicated. The phosphorylation of Elk1 and Atf2 decreased in a dose-dependent manner.

image 2 :

Alomone LabsQO-40enhances KCNQ2/KCNQ3heteromeric channels expressed inXenopusoocytes. - A. Time course of KCNQ2/KCNQ3 current enhancement by 40 and 100 MQO-40(#Q-265). Currents were elicited by application of voltage step from a holding potential of -100 mV to -60 mV (800 msec). B. Superimposed example traces of current responses before and during perfusion of 40 and 100 M QO-40 as indicated.

image 3 :

product information

CAT :

S-370

SKU :

S-370_1 mg

Product Name :

SB 203580

Group Type :

Non Antibodies

Product Type :

S. Molecules

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

SB203580 is highly selective and cell permeable pyridinyl imidazole that inhibits the activity of MAP kinase homologs - p38 (stress-activated protein kinase II/HOG1) and p54 (stress-activated protein kinase I/c-Jun N-terminal kinase) with IC50 values of 50 and 500 nM for p38/SAPK2a and p38/SAPK2b respectively.1-6 SB203580 also inhibits the phosphorylation and activation of protein kinase B (PKB, also known as Akt) by inhibiting the PKB kinase, phosphoinositide-dependent protein kinase 1 (IC50 3-5 µM).7-9

Supplier :

Alomone Labs

Target :

MAPK Homologue-p38

Long Description :

SB 20358 (#S-370) is a highly pure, synthetic, and biologically active compound.

Short Description :

A MAPK Homologue-p38 Inhibitor

MW :

377.44

Source :

Synthetic

Molecular formula :

C21H16FN3OS.

Effective Concentration :

10-100 µM.

Activity :

SB 203580 is a specific inhibitor for the p38 MAP kinase homologues1-6.

Reconstitution and Solubility :

DMSO. Centrifuge all product preparations before use (10000 x g 5 min).

Solubility :

DMSO. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions :

Up to two weeks at 4°C or six months at -20°C.Protect from light.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>99%

CAS No :

152121-47-6

Form :

Lyophilized powder.

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

4-[5-(4-Fluorophenyl)-2-[4-(methylsulfonyl)phenyl]- 1H-imidazol-4-yl]pyridine.

more info or order :

Alomone Labs product webpage