Staurosporine | Alomone Labs S-350 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

Staurosporine

catalog :

S-350

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Ferreira A, Casamento A, Carrillo Roas S, Halff E, Panambalana J, Subramaniam S, et al. Cdk5 and GSK3β inhibit fast endophilin-mediated endocytosis. Nat Commun. 2021;12:2424 pubmed publisher
Hawkins B, Sykes D, Miller D. Rapid, reversible modulation of blood-brain barrier P-glycoprotein transport activity by vascular endothelial growth factor. J Neurosci. 2010;30:1417-25 pubmed publisher
Popovic Z, Sandhoff R, Sijmonsma T, Kaden S, Jennemann R, Kiss E, et al. Sulfated glycosphingolipid as mediator of phagocytosis: SM4s enhances apoptotic cell clearance and modulates macrophage activity. J Immunol. 2007;179:6770-82 pubmed

image

image 1 :

Alomone Labs Staurosporine induces apoptosis in Jurkat cells. - Cells were grown to 70% confluency then 2 MStaurosporine(#S-350)or vehicle were added for 3 or 6 hours. At the end of the incubation period cell proteins were resolved by SDS-PAGE and probed with Caspase 3 and cleaved Caspase 3 specific antibodies.

image 2 :

image 3 :

Alomone Labs Staurosporine inhibits MARCKS phosphorylation via PKC in C6 glioma cells. - Cells were grown to 70% confluence and then serum starved for 18 h. The cells were preincubated for 30 min with different concentrations of Staurosporine (#S-350) and stimulated with 2 M PMA (#P-800) for 30 min. Cell proteins were resolved by SDS-PAGE and probed with antiphospho (ser)-MARCKS (myristoylated alanin-rich protein kinase C substrate).

product information

CAT :

S-350

SKU :

S-350_0.1 mg

Product Name :

Staurosporine

Group Type :

Non Antibodies

Product Type :

S. Molecules

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

Staurosporine is a member of the K252a family of fungal alkaloids produce by Streptomyces staurospores.1,2 It is one of the most potent, cell permeable protein kinase inhibitors, and it is often used to study the involvement of protein kinases in signal transduction pathways (IC50 0.7-20 nM).3-10In pheochromocytoma PC12 cells, neuroblastoma and brain primary culture Staurosporine is a functional neurotropins agonist promoting neurite outgrowth (EC50 = 50 nM).11-12 At concentrations higher then 0.5 mM, Staurosporine generates apoptosis in several different cell types or induces G1 and G2 phase arrest in normal cells.13-16

Supplier :

Alomone Labs

Target :

PKA, PKC, PKG

Long Description :

Staurosporine (#S-350) is a highly pure, natural, and biologically active compound.

Short Description :

A Potent Inhibitor of Protein Kinase A, Protein Kinase C, and Protein Kinase G

MW :

466

Synonyms :

Antibiotic AM-2282

Source :

Natural

Molecular formula :

C28H26N4O3.

Effective Concentration :

10 nM - 1 µM.

Activity :

Staurosporine potently inhibits a variety of kinases including PKC (IC50 = 0.7 nM), PKA (IC50 = 7 nM) and PKG (IC50 = 8.5 nM)1,2. It also inhibits CaMK (IC50 = 20 nM)3 and MLCK (IC50 = 1.3 nM)1,2. In addition, Staurosporine can act as an agonist promoting neurite outgrowth4. At higher concentrations, Staurosporine induces apoptosis5.

Reconstitution and Solubility :

DMSO or methanol. Centrifuge all product preparations before use (10000 x g 5 min).

Solubility :

DMSO or methanol. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions :

Up to one week at 4°C or three months at -20°C.Protect from light.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>98%

CAS No :

62996-74-1

Form :

Lyophilized powder.

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

[9S-(9a,10b,11b,13a)]-2,3,10,11,12,13-Hexahydro-10- meth oxy-9-methyl-11-(methylamino)-9,13-epoxy-1H,9H-diindolo [1,2,3- gh:3',2',1'-lm]pyrrolo[3,4-j][1,7]benzodiazonin-1-one.

more info or order :

Alomone Labs product webpage