chemical

SKF 96365 hydrochloride

S-175

S-175_10 mg

SKF 96365 hydrochloride

Non Antibodies

S. Molecules

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

SKF 96365 hydrochloride is a synthetic blocker of CaV3 (T-type) voltage gated calcium channels and of TRPC channels. In addition, it has a blocking effect on potassium channels. SKF also inhibits the calcium sensor STIM1 which enables Store-operated Ca2+ entry (SOCE). SKF has an effective concentration of 1-100 µM and an IC50 of 560 nM for CaV3.1 channel. SKF is used to identify CaV3 and TRPC channels in many cell types. In an experiment examining the effect of SKF on CaV3 conductivity, perfusion of 1 µM of SKF reversibly inhibited 86.3±0.1% of CaV3.1 currents generated in HEK293 cells. Maximum inhibition was reached within 6-7 minutes. Application of larger concentrations of SKF completely abolished CaV3.1 channel currents. SKF blocks the CaV3.1 channel in a tonic manner indicating it interacts with the channel regardless of its functional state, unlike other CaV channel blockers. In the same experiment SKF also abolished calcium currents in other types of CaV channels such as P/Q-type and N-type.Blockade of these channels was only partially reversible due to possible irreversible binding of or longer washing time required. In addition, SKF blocks L-type calcium channels but in a much slower manner than other calcium channels.

Alomone Labs

CaV3 channels, TRPC, STIM1

SKF 96365 hydrochloride (#S-175) is a highly pure, synthetic, and biologically active compound.

A Blocker of CaV3 and TRPC channels and STIM1

402.9

Synthetic

C22H27ClN2O3.

1-100 µM.

SKF 96365 is an Inhibitor of TRPC channels and inhibitor of T-type calcium channels with an IC50 560 nM for CaV3.1 channels1.

100 mM in H2O. Centrifuge all product preparations before use (10 000 x g for 1 min).

100 mM in H2O. Centrifuge all product preparations before use (10 000 x g for 1 min).

Up to four weeks at 4°C or three months at -20°C.

1-2 Business Days

Israel/IL

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

>98%

130495-35-1

Lyophilized powder.

Contact Alomone Labs for technical support and product customization

41116134

Yes

1-[2-(4-methoxyphenyl)-2-[3-(4-methoxyphenyl)propoxy]ethyl]imidazole;hydrochloride.