Maurocalcine | Alomone Labs RTM-100 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

Maurocalcine

catalog :

RTM-100

more info or order :

Alomone Labs product webpage

image

image 1 :

Alomone Labs Maurocalcine evokes long lasting Ca2+release events in skeletal myotubes. - Myotube cell cultures were prepared from limbs of 2 days old rat pups were loaded with 4 mM fluo-3 AM and then stimulated withMaurocalcine(#RTM-100). Ca2+spreads in the same myotube before (A) and after (B) stimulation with 200 nM Maurocalcine.

image 2 :

Alomone LabsOlmesartaninhibits the activation of Angiotensin AT1R expressed in CHO-K1-mt Aequorin-G?16 cells. - Dose response curve for the inhibition of AT1R expressed in CHO-K1-mt Aequorin-G?16 cells. Ca2+response as detected by elevation in aequorin derived fluorescence following 0.04 nM Angiotensin II application was inhibited by increasing concentrations ofOlmesartan(#O-125). 1 M Olmesartan fully inhibited the activation by Angiotensin II.

product information

cat :

RTM-100

SKU :

RTM-100_0.1 mg

Product Name :

Maurocalcine

Group Type :

Non Antibodies

Product Type :

Proteins

Accession :

P60254

Accession Number :

https://www.uniprot.org/uniprotkb/P60254/entry

Applications :

Calcium imaging assay

Formulation :

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Reconstitution and Solubility :

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Storage Before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Origin :

Scorpio maurus palmatus (Israeli golden scorpion)

Source :

Recombinant, E. coli

Gene ID :

RYR1,RYR2,RYR3

Product Page - Scientific background :

Maurocalcine (MCa) is a peptidyl toxin originally isolated from the venom of the scorpion Scorpio maurus palmatus.Native MCa is present at a low concentration in the venom (0.5% of total protein). This 33-mer basic peptide contains three disulfide bridges and has 82% sequence homology to Imperatoxin A (IpTxa), a scorpion toxin from the venom of Pandinus imperator. MCa shares the highly basic structural domain identified in peptide A - a segment of the dihydropyridine receptor and IpTxa which is important for interactions with ryanodine receptors. MCa also appears to share the same features as the so-called cell-penetrating peptides which allows the toxin to efficiently penetrate various cell types without expending metabolic energy or using an endocytotic mechanisM1-4In vitro, electrophysiological experiments based on recordings of single channels incorporated into planar lipid bilayers showed that MCa potently and reversibly modifies channel gating behavior of the ryanodine receptor 1 (RyR1) by inducing prominent subconductance behavior.1,2Similar to IpTxa, MCa-modified channels can reversibly shuttle between subconductance states and fast gating states. The actions of these peptides were also found to be additive with those of ryanodine, resulting in additional sub-states along with the ryanodine-locked half-conducting state. However, the predominant subconductance induced by MCa differs from that of IpTxa. At a holding potential of +40 mV, the predominant substates induced by sMCa and IpTxa are 48 and 28% of the full conductance, respectively, suggesting that the structural difference in these peptides may induce slightly different channel conformations that stabilize different unitary conductances.5

Supplier :

Alomone Labs

Target :

Ryanodine receptors

Long Description :

A Ryanodine Receptor 1, 2 & 3 Modulator

Short Description :

A Ryanodine Receptor 1, 2 & 3 Modulator

MW :

3858.6 Da

Synonyms :

MCa, Maurocalcin

Modifications :

Disulfide bonds between: Cys3-Cys17, Cys10-Cys21 and Cys16-Cys32

Molecular formula :

C156H270N56O46S6

Effective Concentration :

200 - 400 nM

Activity :

Maurocalcine is an agonist of Ryanodine receptor channel (RyR) types 1' 2 and 3.

Storage of solutions :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Purity :

≥98% (HPLC)

CAS No :

269745-22-4

Form :

Lyophilized

Comment :

Contact Alomone Labs for technical support and product customization

Sequence :

GDCLPHLKLCKENKDCCSKKCKRRGTNIEKRCR-OH

Is Toxin :

Yes

UNSPSC :

12352202

Bioassay Tested :

yes

Steril endotoxin free :

more info or order :

Alomone Labs product webpage