Rabbit IgG Isotype Control-mFluor™ Violet 450 | Alomone Labs RIC-001-V product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Rabbit IgG Isotype Control-mFluor™ Violet 450

catalog :

RIC-001-V

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

application :

flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Establishing the effectiveness of the Rabbit IgG Isotype Control-mFluor™ Violet 450 (#RIC-001-V) control alongside cell surface detection of β1-Adrenergic Receptor by direct flow cytometry in a live intact mouse J774 macrophage cell line. (A) In the absence of an Fc block. (B) In the presence of an Fc block:

product information

CAT :

RIC-001-V

SKU :

RIC-001-V_50 mcl

Product Name :

Rabbit IgG Isotype Control-mFluor™ Violet 450

Group Type :

Antibodies

Product Type :

Isotype Control

Clonality :

Polyclonal

Accession :

Applications :

Reactivity :

Human Mouse Rat

Host :

Rabbit

Homology :

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Label :

mFluor™ Violet 450. Maximum absorption 405 nm and maximum emission 445 nm. These spectral characteristics make it an excellent replacement for Pacific Blue™ or Brilliant Violet™ 421 dyes. For flow cytometry applications, the labeled antibody can be detected using the 405 nm laser lane and a filter set of 450/50.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Sequence :

Product Page - Scientific background :

Applications may also work in :

Supplier :

Alomone Labs

Target :

Short Description :

A Rabbit isotype control antibody conjugated to the fluorescent dye mFluor™ Violet 450 .

Long Description :

Isotype controls are primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in the application. Isotype controls are used as negative controls to help differentiate non-specific background signal from specific antibody signal. Our Rabbit IgG Isotype Control-mFluor™ Violet 450 (#RIC-001-V), was specifically developed to be used as a negative control in surface staining flow cytometry along our line of rabbit primary surface staining antibodies conjugated to mFluor™ Violet 450.

Negative Control :

Positive Control :

Synonyms :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

AB Specifictiy :

The IgG fraction was purified form non-immunized rabbits.

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Serum from non-immunized rabbits was pooled and the IgG fraction was purified on a Protein A column.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Flow cytometry

Antigen preadsorption control :

Application Dilutions: Western blot wb :

more info or order :

Alomone Labs product webpage