Rabbit IgG Isotype Control-ATTO Fluor-488 | Alomone Labs RIC-001-AG product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Rabbit IgG Isotype Control-ATTO Fluor-488

catalog :

RIC-001-AG

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

application :

immunohistochemistry, flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Comparison of immunostaining with P2RY1 compared to immunostaining with isotype control in rat spinal cord

image 2 :

Establishing the effectiveness of the Rabbit IgG Isotype Control-ATTO Fluor-488 (#RIC-001-AG), alongside cell surface detection of P2RX7 by direct flow cytometry in a live intact human THP-1 monocytic leukemia cell line. (A) In the absence of an Fc block. (B) In the presence of an Fc block:

image 3 :

Establishing the effectiveness of the Rabbit IgG Isotype Control-ATTO Fluor-488 (#RIC-001-AG) alongside cell surface detection of CXCR4 by direct flow cytometry in live intact mouse BV-2 microglia cell line:

product information

CAT :

RIC-001-AG

SKU :

RIC-001-AG_50 mcl

Product Name :

Rabbit IgG Isotype Control-ATTO Fluor-488

Group Type :

Antibodies

Product Type :

Isotype Control

Clonality :

Polyclonal

Accession :

Applications :

FC IHC

Reactivity :

Host :

Rabbit

Blocking Peptide :

BLP-ZT024

Homology :

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Label :

ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range. This label is analogous to the dye fluorescein isothiocyanate (FITC) and can be used with filters used to detect FITC.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Sequence :

Product Page - Scientific background :

Applications may also work in :

FC IHC

Supplier :

Alomone Labs

Target :

Short Description :

A Rabbit isotype control antibody conjugated to the fluorescent dye ATTO Fluor-488.

Long Description :

Isotype controls are primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in the application. Isotype controls are used as negative controls to help differentiate non-specific background signal from specific antibody signal. Our Rabbit IgG Isotype Control-ATTO Fluor-488 (#RIC-001-AG), was specifically developed to be used as a negative control in immunohistochemistry and surface staining flow cytometry applications, along our line of rabbit primary antibodies conjugated to the ATTO Fluor-488 fluorophore.

Negative Control :

Positive Control :

Synonyms :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

AB Specifictiy :

The IgG fraction was purified from non-immunized rabbits.

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Serum from non-immunized rabbits was pooled and the IgG fraction was purified on a Protein A column.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Flow cytometry

Antigen preadsorption control :

Application Dilutions: Immunohistochemis ... :

The isotype control antibody should be used at the exact same dilution of the experimental antibody

Application Dilutions: Western blot wb :

more info or order :

Alomone Labs product webpage