chemical

Radicicol

R-370

R-370_0.5 mg

Radicicol

Non Antibodies

S. Molecules

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

An antifungal macrocyclic antibiotic, originally recognized as a protein tyrosine kinase inhibitor. Radicicol inhibits p60v-src kinase activity, and tyrosine phosphorylation of p53/56lyn.1,2 The specific inhibition of the chaperone Hsp90 confers anticancer properties to Radicicol.3,4 In eukaryotic cells, Hsp90 catalyzes the final activation step of many regulatory proteins. Cells that lose Hsp90 function are severely compromised and cannot progress through the cell cycle. Radicicol, similarly to Geldanamycin, binds to the nucleotide-binding pocket, and inhibits ATPase activity of Hsp90.5 This causes destabilization of Hsp90 client proteins (v-Src, Raf-1, ErbB2 Ras), many of which are essential for tumor cell growth.6 Oncogene induced transformation of NIH 3T3 cells (src, ras, and mos) is suppressed by radicicol and it also disrupts the K-Ras-activated signaling pathways by selective depletion of Raf kinase.7,8 In addition, it also exhibits anti-angiogenic activity in vivo, and reduces the expression of VEGF.9,10 Radicicol inhibits AP-1-, NF-kB- and serum response factor (SRF)-mediated transcription.11 It inhibits the expression of COX-2 without affecting COX-1 expression in LPS-stimulated macrophages,1 and induces the differentiation of HL-60 cells into macrophages, by blocking the cell cycle at G1 and G2 sites.12

Alomone Labs

Radicicol (#R-370) is a highly pure, natural, and biologically active compound.

An Anticancer Antibiotic

364.78

Monorden

Natural

C18H17ClO6.

1-100 nM.

Radicicol is an antifungal antibiotic originally discovered to be a tyrosine kinase inhibitor1. The specific inhibition of the chaperone Hsp90 confers anticancer properties to Radicicol2,3. In addition, it also exhibits anti-angiogenic activity in vivo, and reduces the expression of VEGF4,5.

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity

DMSO, ethanol. Centrifuge all product preparations before use (10000 x g 5 min).

Up to four weeks at 4°C or three months at -20°C.

1-2 Business Days

Israel/IL

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

>99%

12772-57-5

Lyophilized powder.

Contact Alomone Labs for technical support and product customization

41116134

Yes

(1aR,2Z,4E,14R,15aR)-8-Chloro-1a,14,15,15atetrahydro-9 ,11-dihydroxy-14-methyl-6H-oxireno[e][2]benzoxacyclotetradecin-6,12(7H)-dione.