chemical

RO-3

R-190

R-190_10 mg

RO-3

Non Antibodies

S. Molecules

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

RO-3 is a potent inhibitor of human homomultimeric P2X3 receptor and heteromultimeric P2X2/3 receptor and demonstrates pIC50 value of 7.0 and pIC50=5.9 respectively1.The compound shows to have to high metabolic stability in rat and human hepatocytes and liver microsomes. It's highly permeable, orally bioavailable, and has a good in vivo plasma half-life in rats. RO-3 is also widely distributed to tissues after administration, with low plasma protein binding and good CNS penetration1.The P2X receptors are a family of ion channels that are gated by ATP. P2X receptors are widely localized in cell types of almost every origin, including neuronal, muscular, epithelial, and immune and have been shown to play a pivotal role in models of various pain conditions2.

Alomone Labs

P2X3 receptors

RO-3 (#R-190) is a highly pure, synthetic, and biologically active compound.

A Potent and Selective Antagonist of P2X3 and P2X2/3 Receptors

302.37

Synthetic

C16H22N4O2.

50 nM - 50 µM.

RO-3 is a potent and selective antagonist of the P2X3 and P2X2/3 receptors. It inhibits human homomeric P2X3 and heteromeric P2X2/3 receptors with pIC50 values of 7.0 and 5.9, respectively, whilst showing selectivity over other homomeric P2X family receptors with pIC50 of < 51.

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity

Up to 100 mM in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).

-20°C.

1-2 Business Days

Israel/IL

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

>97% (HPLC)

1026582-88-6

Lyophilized powder.

Contact Alomone Labs for technical support and product customization

41116134

Yes

5-[(4,5-dimethoxy-2-propan-2-ylphenyl)methyl]pyrimidine-2,4-diamine.