chemical

Ro46-8443

R-185

R-185_1 mg

Ro46-8443

Non Antibodies

S. Molecules

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Ro46-8443 is a synthetic compound that acts as a selective and competitive antagonist of the ET-B (endothelin B) receptor, a receptor that belongs to the G-protein coupled receptor (GPCR) superfamily. Ro46-8443 shows a 100-fold selectivity for human ET-B over ET-A receptors and is being used as an important tool for studying the role of ET-B receptors in pathology1. Ro 46-8443 demonstrates IC50 values of 34 nM and 69 nM in competitive binding assays for human ET-B receptors in radiolabeled membranes of Chinese hamster ovary cells and microsomal membranes from human placenta, respectively. The compound also inhibits the binding of selective radiolabeled ET-B agonist Sarafotoxin-S6c to porcine trachea membranes with an IC50 value of 4.9 nM1.Endothelin receptors are widely distributed in vascular and nonvascular tissues. The ET-B receptor has high and equal affinity for all endothelin isopeptides3. ET-B receptors play an important role in regulating renal function and blood pressure and are expressed in sensory nerves. They are mainly present in medium- and large-sized cell bodies of human trigeminal ganglia3.

Alomone Labs

ET-B receptor

Ro46-8443 (#R-185) is a highly pure, synthetic, and biologically active compound.

A Selective Antagonist of Endothelin B Receptors

609.69

Synthetic

C31H35N3O8S.

0.1-10 µM.

Ro 46-8443 is a non-peptide, selective and competitive antagonist of ET-B receptors. It shows over 100-fold selectivity for human ET-B in competitive binding assays (IC50 of 34-69 nM), and competitively inhibits the binding of selective ET-B agonist [125I]Sarafotoxin-S6c to porcine trachea membranes (IC50 = 4.9 nM)1.

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity

Up to 100 mM in DMSO. Centrifuge all products before use (10000 x g 5 min).

Up to three months at -20°C.

1-2 Business Days

Israel/IL

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

>95%

175556-12-4

Lyophilized powder.

Contact Alomone Labs for technical support and product customization

41116134

Yes

4-tert-butyl-N-[6-[(2R)-2,3-dihydroxypropoxy]-5-(2-methoxyphenoxy)-2-(4-methoxyphenyl)pyrimidin-4-yl]benzenesulfonamide.