chemical

QO-40

Q-265

Q-265_10 mg

QO-40

Non Antibodies

S. Molecules

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

QO-40 potently enhances KCNQ channels. It has the ability to decrease the firing of overactive neurons. In medicine, this compound is being used for the treatment of epilepsy and neuropathic pain. In a study conducted on Chinese hamster ovary cells, QO-40 managed to shift the half-maximal activation voltage in the hyperpolarizing direction in a concentration-dependent manner1. QO-40 demonstrates EC50 value of approximately 7 µM on cloned channels and on native M-currents expressed in dorsal root ganglia neurons1.The voltage-gated M-type potassium channels of the KV7 family encoded by the KCNQ gene family, plays an important role in controlling neuronal excitability. KCNQ gene family seems to affect migraine and neuropathic pain. Several recent studies have shown that genetic mutations in KCNQ channel can lead to neonatal epilepsy in humans and mice.KCNQ channels are expressed in the sensory system including the trigeminal ganglion neurons and dorsal root ganglion2,3.

Alomone Labs

KCNQ channels

QO-40 (#Q-265) is a highly pure, synthetic, and biologically active compound.

An Opener of KCNQ Channels

377.8

Synthetic

C18H11ClF3N3O.

1-100 µM.

QO-40 is a KCNQ channel opener with EC50 of ~7 µM on cloned channels and on native M-current in DRG neurons1.

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity

200 mM in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).

Up to four weeks at 4°C or three months at -20°C.

1-2 Business Days

Israel/IL

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

>99%

1259536-70-3

Lyophilized Powder

Contact Alomone Labs for technical support and product customization

41116134

Yes

5-(chloromethyl)-3-(naphthalen-1-yl)-2-(trifluoromethyl)pyrazolo[1,5-a]pyrimidin-7(4H)-one.