QX-314 bromide | Alomone Labs Q-100 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

QX-314 bromide

catalog :

Q-100

more info or order :

Alomone Labs product webpage

citations: 37

Reference
Chowdhury S, Hung C, Izawa S, Inutsuka A, Kawamura M, Kawashima T, et al. Dissociating orexin-dependent and -independent functions of orexin neurons using novel Orexin-Flp knock-in mice. elife. 2019;8: pubmed publisher
Stötzner P, Spahn V, Celik M, Labuz D, Machelska H. Mu-Opioid Receptor Agonist Induces Kir3 Currents in Mouse Peripheral Sensory Neurons - Effects of Nerve Injury. Front Pharmacol. 2018;9:1478 pubmed publisher
Marcoux C, Clarke S, Nesse W, Longtin A, Maler L. Balanced ionotropic receptor dynamics support signal estimation via voltage-dependent membrane noise. J Neurophysiol. 2016;115:530-45 pubmed publisher
Suvrathan A, Bennur S, Ghosh S, Tomar A, Anilkumar S, Chattarji S. Stress enhances fear by forming new synapses with greater capacity for long-term potentiation in the amygdala. Philos Trans R Soc Lond B Biol Sci. 2014;369:20130151 pubmed publisher
Scheuss V, Bonhoeffer T. Function of dendritic spines on hippocampal inhibitory neurons. Cereb Cortex. 2014;24:3142-53 pubmed publisher
Ye Z, Li D, Li L, Pan H. Protein kinase CK2 increases glutamatergic input in the hypothalamus and sympathetic vasomotor tone in hypertension. J Neurosci. 2011;31:8271-9 pubmed publisher
Sanon N, Pelletier J, Carmant L, Lacaille J. Interneuron subtype specific activation of mGluR1/5 during epileptiform activity in hippocampus. Epilepsia. 2010;51:1607-18 pubmed publisher
Marchionni I, Takács V, Nunzi M, Mugnaini E, Miller R, Maccaferri G. Distinctive properties of CXC chemokine receptor 4-expressing Cajal-Retzius cells versus GABAergic interneurons of the postnatal hippocampus. J Physiol. 2010;588:2859-78 pubmed publisher
Drew G, Lau B, Vaughan C. Substance P drives endocannabinoid-mediated disinhibition in a midbrain descending analgesic pathway. J Neurosci. 2009;29:7220-9 pubmed publisher
Cohen D, Segal M. Homeostatic presynaptic suppression of neuronal network bursts. J Neurophysiol. 2009;101:2077-88 pubmed publisher
Pilpel Y, Kolleker A, Berberich S, Ginger M, Frick A, Mientjes E, et al. Synaptic ionotropic glutamate receptors and plasticity are developmentally altered in the CA1 field of Fmr1 knockout mice. J Physiol. 2009;587:787-804 pubmed publisher
Watanabe K, Kita T, Kita H. Presynaptic actions of D2-like receptors in the rat cortico-striato-globus pallidus disynaptic connection in vitro. J Neurophysiol. 2009;101:665-71 pubmed publisher
Jeong H, Chenu D, Johnson E, Connor M, Vaughan C. Sumatriptan inhibits synaptic transmission in the rat midbrain periaqueductal grey. Mol Pain. 2008;4:54 pubmed publisher
Zhou H, Chen S, Chen H, Pan H. The glutamatergic nature of TRPV1-expressing neurons in the spinal dorsal horn. J Neurochem. 2009;108:305-18 pubmed publisher
Hermann J, Grothe B, Klug A. Modeling short-term synaptic plasticity at the calyx of Held using in vivo-like stimulation patterns. J Neurophysiol. 2009;101:20-30 pubmed publisher
Abrahamsson T, Gustafsson B, Hanse E. AMPA silencing is a prerequisite for developmental long-term potentiation in the hippocampal CA1 region. J Neurophysiol. 2008;100:2605-14 pubmed publisher
Olijslagers J, de Kloet E, Elgersma Y, van Woerden G, Joels M, Karst H. Rapid changes in hippocampal CA1 pyramidal cell function via pre- as well as postsynaptic membrane mineralocorticoid receptors. Eur J Neurosci. 2008;27:2542-50 pubmed publisher
Xing J, Li D, Li J. Role of GABA receptors in nitric oxide inhibition of dorsolateral periaqueductal gray neurons. Neuropharmacology. 2008;54:734-44 pubmed publisher
Graciotti L, Minelli A, Minciacchi D, Procopio A, Fulgenzi G. GABAergic miniature spontaneous activity is increased in the CA1 hippocampal region of dystrophic mdx mice. Neuromuscul Disord. 2008;18:220-6 pubmed publisher
Abrahamsson T, Gustafsson B, Hanse E. Reversible synaptic depression in developing rat CA3 CA1 synapses explained by a novel cycle of AMPA silencing-unsilencing. J Neurophysiol. 2007;98:2604-11 pubmed
Fujiwara Tsukamoto Y, Isomura Y, Imanishi M, Fukai T, Takada M. Distinct types of ionic modulation of GABA actions in pyramidal cells and interneurons during electrical induction of hippocampal seizure-like network activity. Eur J Neurosci. 2007;25:2713-25 pubmed
Zhang H, Chen S, Pan H. Regulation of glutamate release from primary afferents and interneurons in the spinal cord by muscarinic receptor subtypes. J Neurophysiol. 2007;97:102-9 pubmed
Tu B, Jiao Y, Herzog H, Nadler J. Neuropeptide Y regulates recurrent mossy fiber synaptic transmission less effectively in mice than in rats: Correlation with Y2 receptor plasticity. Neuroscience. 2006;143:1085-94 pubmed
Boddy G, Willis A, Galante G, Daniel E. Sodium-, chloride-, and mibefradil-sensitive calcium channels in intestinal pacing in wild-type and W/WV mice. Can J Physiol Pharmacol. 2006;84:589-99 pubmed
Chen Q, Pan H. Regulation of synaptic input to hypothalamic presympathetic neurons by GABA(B) receptors. Neuroscience. 2006;142:595-606 pubmed
Karst H, Berger S, Turiault M, Tronche F, Schutz G, Joels M. Mineralocorticoid receptors are indispensable for nongenomic modulation of hippocampal glutamate transmission by corticosterone. Proc Natl Acad Sci U S A. 2005;102:19204-7 pubmed
Abrahamsson T, Gustafsson B, Hanse E. Synaptic fatigue at the naive perforant path-dentate granule cell synapse in the rat. J Physiol. 2005;569:737-50 pubmed
Karst H, Joels M. Corticosterone slowly enhances miniature excitatory postsynaptic current amplitude in mice CA1 hippocampal cells. J Neurophysiol. 2005;94:3479-86 pubmed
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image

image 1 :

Alomone Labs QX-314 bromideinhibits native NaVchannels in ND7-23 cells. - Currents were elicited from a holding potential of -100 mV and test pulses of 40 ms to -10 mV were delivered every 15 sec. Superimposed traces of control currents (black) and during a bath perfusion of 2.5 mM (blue) and 25 mM (orange)QX-314 bromide(#Q-100).

product information

CAT :

Q-100

SKU :

Q-100_1 g

Product Name :

QX-314 bromide

Group Type :

Non Antibodies

Product Type :

S. Molecules

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

This local anesthetic is a blocker of voltage-dependent Na+ channels. QX-314 blocks all NaV channels once exposed to the intracellular face of the channel protein. However, this compound can penetrate the cell interior once applied outside the cell. Via certain types of NaV channels, it can permeate and exert blocking activity via TTX-sensitive channels.1 Intracellular recordings from inferior olivary neurons in brain stem slice preparations demonstrated that the Na+-dependent action potential was completely blocked for approximately five minutes after impalement with a microelectrode containing 50 mM QX-314, whereas the low-threshold Ca2+ spike was still generated.2 QX-314 is widely used to block NaV channels and used as a tool in neurophysiological recordings.2-5

Supplier :

Alomone Labs

Target :

Various NaV Na+ channels

Long Description :

QX-314 bromide (#Q-100) is a highly pure, synthetic, and biologically active compound.

Short Description :

A Local Anesthetic Blocker of Some NaV Channels

MW :

343.3

Synonyms :

Lidocaine N-ethyl bromide

Source :

Synthetic

Molecular formula :

C16H27N2OBr.

Effective Concentration :

1-50 mM.

Activity :

QX-314 bromide is an intracellular blocker of NaV channels1-4.

Reconstitution and Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity

Solubility :

Water. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions :

Up to two weeks at 4°C or six months at -20°C. Protect from light. Hygroscopic.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>99% (HPLC)

CAS No :

21306-56-9

Form :

Lyophilized powder.

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Cited Application :

Electrophysiology

Chemical Name :

N-(2,6-Dimethylphenylcarbamoylmethyl) triethylammonium bromide.

more info or order :

Alomone Labs product webpage