chemical

PMA

P-800

P-800_1 mg

PMA

Non Antibodies

S. Molecules

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Phorbol 12-myristate 13-acetate (PMA) is the most commonly-used phorbol ester, primary found as extremely potent tumor promoter.1-3 PMA is a reversible activator of Protein Kinase C (PKC)4 in vivo and in vitro, even at nM concentrations (cells are usually treated with PMA at 200 nM to induce signaling). PMA promotes the expression of inducible NOS in cultured hepatocytes, activates Ca2+-ATPase pump and potentates forskolin-induced cAMP formation. In some cases PMA inhibits apoptosis induced by the Fas antigen, but induces apoptosis in HL-60 promyelocytic leukemia cells.5-8

Alomone Labs

PKC

PMA (#P-800) is a highly pure, semi-synthetic, and biologically active compound.

A PKC Activator

616.84

Phorbol 12-myristate 13-acetate, TPA, 12-O-tetradecanoylphorbol-13-acetate

Semi-synthetic

C36H56O8.

200 nM.

PMA is a commonly used phorbol ester and activates PKC in nM concentrations1. It has also been shown to be an extremely potent tumor promoter2-4.

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity

DMSO or methanol. Centrifuge all product preparations before use (10000 x g 5 min).

Up to four weeks at 4°C or three months at -20°C.Highly irritable to skin and mucous membranes. Tumor promoter. Gloves and mask should be worn when using this product.

1-2 Business Days

Israel/IL

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

>99.5%

16561-29-8

Lyophilized powder.

Contact Alomone Labs for technical support and product customization

41116134

Yes

(1aR,1bS,4aR,7aS,7bS,8R,9R,9aS)-9a-(Acetyloxy)- 1a,1b,4, 4a,5,7a,7b,8,9,9a-decahydro-4a,7b-dihydroxy-3-(hydroxymethyl)-1,1,6,8-tetramethyl-5-oxo-1H-cyclopropa[3,4]benz [1,2-e]azulen-9-yl tetradecanoate.