Penitrem A | Alomone Labs P-650 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

Penitrem A

catalog :

P-650

more info or order :

Alomone Labs product webpage

citations: 7

Reference
Irie T. Essential Role of Somatic Kv2 Channels in High-Frequency Firing in Cartwheel Cells of the Dorsal Cochlear Nucleus. Eneuro. 2021;8: pubmed publisher
Yang M, Dart C, Kamishima T, Quayle J. Hypoxia and metabolic inhibitors alter the intracellular ATP:ADP ratio and membrane potential in human coronary artery smooth muscle cells. Peerj. 2020;8:e10344 pubmed publisher
Zavaritskaya O, Dudem S, Ma D, Rabab K, Albrecht S, Tsvetkov D, et al. Vasodilation of rat skeletal muscle arteries by the novel BK channel opener GoSlo is mediated by the simultaneous activation of BK and Kv 7 channels. Br J Pharmacol. 2020;177:1164-1186 pubmed publisher
Rancz E, HÃ¤usser M. Dendritic spikes mediate negative synaptic gain control in cerebellar Purkinje cells. Proc Natl Acad Sci U S A. 2010;107:22284-9 pubmed publisher
Ishii H, Nakajo K, Yanagawa Y, Kubo Y. Identification and characterization of Cs(+) -permeable K(+) channel current in mouse cerebellar Purkinje cells in lobules 9 and 10 evoked by molecular layer stimulation. Eur J Neurosci. 2010;32:736-48 pubmed publisher
Zhou Y, Tang Q, Xia X, Lingle C. Glycine311, a determinant of paxilline block in BK channels: a novel bend in the BK S6 helix. J Gen Physiol. 2010;135:481-94 pubmed publisher
Donato R, Page K, Koch D, Nieto Rostro M, Foucault I, Davies A, et al. The ducky(2J) mutation in Cacna2d2 results in reduced spontaneous Purkinje cell activity and altered gene expression. J Neurosci. 2006;26:12576-86 pubmed

image

image 1 :

Alomone Labs Penitrem A inhibits KCa1.1 (BK) channels heterologously expressed inXenopusoocytes. - The effect of 100 nMPaxilline(#P-450) (a)Penitrem A(#P-650) (b) andVerruculogen(#V-500) (c) on BK currents. Time course of current amplitude changes upon sequential application of the three toxins.

image 2 :

Western blot analysis of rat testis (lanes 1 and 4) and kidney lysates (lanes 2 and 5) and heart membrane (lanes 3 and 6): - 1-3. Anti-MRS2 Antibody(#ANT-148) (1:400).4-6. Anti-MRS2 Antibody preincubated with the negative control antigen.

product information

CAT :

P-650

SKU :

P-650_0.65 mg

Product Name :

Penitrem A

Group Type :

Non Antibodies

Product Type :

S. Molecules

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

Penitrem A is widely used to probe KCa1.1 channels in smooth muscle preparations, where it induces prolongation of the depolarization plateau potential.1 Penitrem A is a selective blocker of KCa1.1 (high-conductance Ca2+-activated K+, BK) channels. It belongs to a set of tremorgenic indole alkaloids which are lipid soluble and also include Verruculogen (#V-500) and Paxilline (#P-450).2 Penitrem A (400 mg mycelium/kg) increased the spontaneous release of endogenous glutamate, GABA (γ-aminobutyric acid), and aspartate by 213%, 455%, and 277%, respectively, from rat cerebrocortical synaptosomes.3

Supplier :

Alomone Labs

Target :

KCa1.1 K+ channels

Long Description :

Penitrem A (#P-650) is a highly pure, natural, and biologically active compound.

Short Description :

A Blocker of KCa1.1 (BK) Channels

MW :

634.2

Synonyms :

Tremortin A

Source :

Natural

Molecular formula :

C37H44ClNO6.

Effective Concentration :

10-100 nM.

Activity :

Penitrem A is a selective blocker of KCa1.1 (high-conductance Ca2+-activated K+, BK) channels1. Penitrem A increases the spontaneous release of endogenous GABA (3-aminobutyric acid), and aspartate2.

Reconstitution and Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity

Solubility :

DMSO, methanol, acetone. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions :

Up to one week at 4°C or three months at -20°C.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>98%

CAS No :

12627-35-9

Form :

Lyophilized powder.

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

(1S,2R,5S,6S,8R,9S,10R,12S,15R,16S,25R,27S,28R)-21-chloro-15,16,33,33-tetramethyl-24-methylidene-10-prop-1-en-2-yl-7,11,32-trioxa-18--]tritriaconta25,28.022,29.019,30.017,31.06,12.06,8.05,15.02,16zadecacyclo[25.4.2.0a17(31),19,21,29-tetraene-5,9,28-triol

more info or order :

Alomone Labs product webpage