PLTX-II | Alomone Labs P-510 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

PLTX-II

catalog :

P-510

more info or order :

Alomone Labs product webpage

citations: 5

Reference
Astorga G, Hartel S, Sanhueza M, Bacigalupo J. TRP, TRPL and cacophony channels mediate Ca2+ influx and exocytosis in photoreceptors axons in Drosophila. PLoS ONE. 2012;7:e44182 pubmed publisher
Chen Z, Wang Q, Wang Z. The amiloride-sensitive epithelial Na+ channel PPK28 is essential for drosophila gustatory water reception. J Neurosci. 2010;30:6247-52 pubmed publisher
Geiser D, Shen M, Mayo J, Winzerling J. Iron loaded ferritin secretion and inhibition by CI-976 in Aedes aegypti larval cells. Comp Biochem Physiol B Biochem Mol Biol. 2009;152:352-63 pubmed publisher
Worrell J, Levine R. Characterization of voltage-dependent Ca2+ currents in identified Drosophila motoneurons in situ. J Neurophysiol. 2008;100:868-78 pubmed publisher
Hartwig C, Worrell J, Levine R, Ramaswami M, Sanyal S. Normal dendrite growth in Drosophila motor neurons requires the AP-1 transcription factor. Dev Neurobiol. 2008;68:1225-42 pubmed publisher

image

image 1 :

Alomone Labs NS-4591 activates KCa3.1 currents expressed in HEK 293T cells. - A. Time course of KCa3.1 current amplitude and its activation by 1 MNS-4591(#N-325). Currents were elicited by application of voltage ramp from -120 mV to +60 mV (150 msec) delivered every 10 seconds holding potential of -80mV. B. Superimposed example traces of current responses before and during perfusion of 1 M NS-4591 as indicated.

product information

cat :

P-510

SKU :

P-510_5 mcg

Product Name :

PLTX-II

Group Type :

Non Antibodies

Product Type :

Proteins

Accession :

P34079

Accession Number :

https://www.uniprot.org/uniprotkb/P34079/entry

Formulation :

Lyophilized Powder.

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Reconstitution and Solubility :

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Storage Before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Origin :

Plectreurys tristis (Spider) (Plectreurys bispinosus)

Source :

Natural peptide

Gene ID :

CACNA, CACNA1B, CACNA1E

Product Page - Scientific background :

Plectreurys toxin (PLTX-II) is a 44 amino acid peptidyl toxin produced by Plectreurys tristis spider1,2. PLTXII was found as a potent blocker of the CaV2 channel (CAC) that inactivates Ca2+ currents in pupal neurons3 and in antennal lobe PNs of adult Drosophila brain. PLTX-II regulates the miniature excitatory postsynaptic current frequency at excitatory cholinergic synapses of these neurons in vitro and in situ and acts as a presynaptic Ca2+-channel blocker3,4.

Supplier :

Alomone Labs

Target :

CaV Ca2+ channels

Long Description :

An Inhibitor of CaV Channels

Short Description :

An Inhibitor of CaV Channels

MW :

5108.7 Da

Synonyms :

ω-Plectoxin-Pt1a, ω-PLTX-Pt1a, PLTX-2

Modifications :

Disulfide bonds between: Cys3-Cys17, Cys10-Cys23, Cys16-Cys35, Cys20-Cys42 and Cys25-Cys33 Thr44 - C-terminal amidation and Opalmitoylation

Molecular formula :

C208H313N61O70S10

Effective Concentration :

10 - 50 nM

Activity :

PLTX-II is a voltage-gated Ca2+ channel blocker.

Storage of solutions :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Purity :

≥99% (HPLC)

Form :

Lyophilized

Comment :

Contact Alomone Labs for technical support and product customization

Sequence :

ADCSATGDTCDHTKKCCDDCYTCRCGTPWGANCRCDYYK
ARCDT-NH2

Is Toxin :

Yes

UNSPSC :

12352202

Steril endotoxin free :

more info or order :

Alomone Labs product webpage