chemical

PNU-120596

P-350

P-350_10 mg

PNU-120596

Non Antibodies

S. Molecules

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

PNU-120596 is as a potent and selective positive allosteric modulator of α7 nicotinic acetylcholine receptors (nAChRs). It potentiates nAChR α7 currents in Xenopus oocytes and in SH-EP1 cells with EC50 values of 1.6 µM and 216 nM, respectively1-2. The compound displays neuroprotective characteristics in vivo and has the ability to increase the potency and maximal efficacy of agonists and to prolong the duration of the agonist-evoked macroscopic currents1,2.nAChRs receptors are responsible for mediating the effects of the neurotransmitter acetylcholine (ACh). They play critical roles in the central and peripheral nervous system where they regulate neurotransmitter release, cell excitability, neuronal integration. They are involved in functions such as sleep and arousal patterns, fatigue, hunger, anxiety, and pain processing2,3,4.

Alomone Labs

α7 nAChR

PNU-120596 (#P-350) is a highly pure, synthetic, and biologically active compound.

A Potent and Selective Positive Allosteric Modulator of α7 nAChR

311.72

Synthetic

C13H14ClN3O4.

0.3-10 µM.

PNU-120596 is a potent and selective type-II positive allosteric modulator of α7 nicotinic acetylcholine receptors, shown to potentiate α7 currents in X. Laevis oocytes and in SH-EP1 cells with EC50 values of 1.6 µM and 216 nM, respectively1-2. Neuroprotective in vivo3.

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity

Soluble to 100 mM in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).

Store at -20°C. It is recommended to prepare solutions afresh on the day of use, or aliquot stock solutions stored at -20°C to prevent repeated thawing and freezing.

1-2 Business Days

Israel/IL

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

>98% (HPLC)

501925-31-1

Lyophilized powder.

Contact Alomone Labs for technical support and product customization

41116134

Yes

1-(5-chloro-2,4-dimethoxyphenyl)-3-(5-methyl-1,2-oxazol-3-yl)urea.