Pimaric Acid | Alomone Labs P-270 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

Pimaric Acid

catalog :

P-270

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Howitt L, Sandow S, Grayson T, Ellis Z, Morris M, Murphy T. Differential effects of diet-induced obesity on BKCa {beta}1-subunit expression and function in rat skeletal muscle arterioles and small cerebral arteries. Am J Physiol Heart Circ Physiol. 2011;301:H29-40 pubmed publisher

image

image 1 :

Alomone Labs Pimaric Acid was tested inXenopusoocytes expressing KCa1.1 channel using TEVC recordings. - Left: Time course of KCa1.1 channel amplitude by applying 50 MPimaric Acid(#P-270) and 200 nMCharybdotoxin(#STC-325) (ChTx). Currents were elicited by a 100 ms pulse to 0 mV from holding potential of -100 mV. Pulses were delivered every 10 seconds. The bars at the top indicate the period of the indicated drug application. Currents were normalized to the values of controls. The red green and blue symbols mark the traces that are shown on the right. Right: Example traces of control (red) Pimaric Acid activated (green) and Charybdotoxin (ChTx) inhibited (blue). KCa1.1 currents are taken from the experiment shown on the left.

image 2 :

product information

CAT :

P-270

SKU :

P-270_1 mg

Product Name :

Pimaric Acid

Group Type :

Non Antibodies

Product Type :

S. Molecules

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

PiMA was found to be a potent opener of KCa1.1 (large conductance Ca2+ activated K+ (BK)) channels. 1 µM PiMA doubled the current carried via BK channels and this effect increased dose dependently. PiMA increases the open probability of single BK channels by shifting both its voltage and Ca2+ dependence to more hyperpolarized voltages.1 BK channels that were activated by PiMA retain their charybdotoxin sensitivity.

Supplier :

Alomone Labs

Target :

KCa1.1 K+ channels

Long Description :

Pimaric Acid (#P-270) is a highly pure, natural, and biologically active compound.

Short Description :

A Novel Opener of KCa1.1 Channels

MW :

302

Synonyms :

PiMA

Source :

Natural

Molecular formula :

C20H30O2.

Effective Concentration :

1-10 µM.

Activity :

Pimaric acid is a potent opener of large conductance Ca2+-activated K+ (BK) channels. 1 µM of Pimaric acid doubles the current carried via BK channels1.

Reconstitution and Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Solubility :

Organic solvents such as DMSO, ethanol, acetone or methanol. DMSO is suggested for biological experiments. DMSO solution should not exceed 0.03%. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions :

Up to three months at -20°C.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>85%

CAS No :

127-27-5

Form :

Lyophilized powder.

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

1-Phenanthrenecarboxylicacid,7-ethenyl-1,2,3,4,4a,4b,5,6,7,9,10,10a-dodecahyde.

more info or order :

Alomone Labs product webpage