chemical

PD 98059

P-260

P-260_1 mg

PD 98059

Non Antibodies

S. Molecules

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

PD 98059 is a synthetic flavonoid and a potent inhibitor of mitogen-activated protein kinase (MAPK) by inhibiting its immediate upstream activator, mitogen activated protein kinase kinase (also known as MEK or MKK1).1-3PD 98059 arrests cell cycle progression of some rat cultured cells at the G0/G1 phase and blocks nerve growth factor stimulated axonal outgrowth from adult mouse dorsal root ganglia in vitro,4-5 and blocks induction of LTP in the brain areas.6-7PD 98059 is also an equipotent antagonist of aryl hydrocarbon receptor (AHR) stimulated by TCDD in MCF10A cells. 8-10

Alomone Labs

Mitogen-Activated Protein Kinase (MAPK)

PD 98059 (#P-260) is a highly pure, synthetic, and biologically active compound.

A Potent Inhibitor of Mitogen-Activated Protein Kinase (MAPK) Pathway

267.28

Synthetic

C16H13NO3.

1-80 µM.

PD 98059 specifically inhibits the mitogen-activated protein kinase kinase (MAPKK) also known as MEK1 and MEK2. It is commonly used in studies of the MAPK pathway.

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes). The lyophilizate may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. Soluble in DMSO. Prepare a concentrated stock solution by dissolving the lyophilized peptide in DMSO first (e.g., at a concentration between 100-1000x of the final working concentration). Once the peptide is completely dissolved in DMSO, slowly dilute the solution into the working buffer (or water) to the desired final working concentration. Centrifuge all product preparations before use. It is recommended to keep the DMSO concentration as low as possible. For cell assays, a final concentration of 0.1%–0.5% DMSO (v/v) is considered safe. For other experiments, a 5% DMSO (v/v) concentration is recommended

DMSO. Centrifuge all product preparations before use (10000 x g 5 min).

Up to two weeks at 4°C or six months at -20°C.Protect from light.

1-2 Business Days

Israel/IL

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

>99%

167869-21-8

Lyophilized powder.

Contact Alomone Labs for technical support and product customization

41116134

Yes

2-(2-Amino-3-methoxyphenyl)-4H-1-benzopyran-4-one.