chemical

Parthenolide

P-250

P-250_25 mg

Parthenolide

Non Antibodies

S. Molecules

Store the reconstituted solution for the shortest time possible at -20°C. We do not recommend storing the product in working solution for longer than one day. Avoid multiple freeze-thaw cycles.

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Parthenolide is a sesquiterpene lactone found in Mexican-Indian medicinal plants such as Tanacetum parthenium. It has been widely used in indigenous medical practices for the treatment of migraine, inflammation and tumors.1-4 Parthenolide inhibits IkB kinase (IKK) and IKKβ (the upstream activators of the Nuclear Factor-kB (NF-kB) pathway), thus inhibiting Interleukin 1- and tumor necrosis factor a (TNFα) without affecting the activation of p38 and c-Jun N-terminal kinase.5,6In breast adenocarcinoma and pancreatic tumor cell lines which constitutively express active NF-κB, parthenolide inhibits NF-κB DNA binding and increases sensitivity to chemotherapeutic agents. The sensitivity of these tumor cells to parthenolide is due to the low expression level of the multifunctional detoxification enzyme glutathione S-transferase p caused by inhibition of NF-κB activity.7,10

Alomone Labs

Parthenolide (#P-250) is a highly pure, natural, and biologically active compound.

A Nuclear Factor-κB (NF-κB) Pathway Inhibitor

248.3

Natural

C15H20O3.

10-50 µM.

Parthenolide is a sesquiterpene lactone and inhibits IκB kinase (IKK) and IKKβ (the upstream activators of the Nuclear Factor-κB (NF-κB) pathway)1,2.

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes). The lyophilizate may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. Soluble in DMSO. Prepare a concentrated stock solution by dissolving the lyophilized peptide in DMSO first (e.g., at a concentration between 100-1000x of the final working concentration). Once the peptide is completely dissolved in DMSO, slowly dilute the solution into the working buffer (or water) to the desired final working concentration. Centrifuge all product preparations before use. It is recommended to keep the DMSO concentration as low as possible. For cell assays, a final concentration of 0.1%–0.5% DMSO (v/v) is considered safe. For other experiments, a 5% DMSO (v/v) concentration is recommended

DMSO, ethanol, methanol. Centrifuge all product preparations before use (10000 x g 5 min).

Up to one week at 4°C or six months at -20°C.

1-2 Business Days

Israel/IL

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

>99%

20554-84-1

Lyophilized powder.

Contact Alomone Labs for technical support and product customization

41116134

Yes

(1aR,7aS,10aS)- 1a,5-dimethyl-8-methylene- 2,3,6,7,7a,8,10a,10b- octahydrooxireno[9,10]cyclodeca [1,2-b]furan-9(1aH)-one.