PEPA | Alomone Labs P-200 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

PEPA

catalog :

P-200

more info or order :

Alomone Labs product webpage

image

image 1 :

Alomone Labs PEPA potentiates GluA1 channels expressed in Xenopus oocytes. - Time course of current reversible potentiation by 250 ?MPEPA(#P-200) on glutamate-elicited currents (100 ?M holding potential -80 mV).

image 2 :

Alomone LabsLY278584blocks 5-HT3A receptors expressed in HEK 293T cells. - 5-HT3A receptor currents were elicited by 10 M 5-HT delivered every 3 minutes.LY278584(#L-180) applied 30 seconds before stimulation at 100 nM inhibited the 5-HT induced current in a dose dependent and reversible manner.

product information

CAT :

P-200

SKU :

P-200_10 mg

Product Name :

PEPA

Group Type :

Non Antibodies

Product Type :

S. Molecules

Accession :

https://www.uniprot.org/uniprotkb/O60542/entry

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

PEPA is a flop splice variant and GluR3/4-prefrerring potentiator of AMPA receptors. PEPA is small chemical compound that enhances AMPA receptor channel activity by modulating receptor kinetics such as desensitization and deactivation. It reportedly enhances synaptic response by selectively acting on AMPA receptors. PEPA (1-200 μM) dose dependently potentiates glutamate-evoked currents in Xenopus oocytes expressing AMPA (GluRA-GluRD). At 200 μM, PEPA could potentiate glutamate responses by 50-fold in oocytes expressing GluRCflop (EC50 ∼50 μM)1. In addition, it potently facilitates extinction learning through activation of AMPA receptors mainly in the medial prefrontal cortex (mPFC)2.

Supplier :

Alomone Labs

Target :

AMPA receptors

Long Description :

PEPA (#P-200) is a highly pure, synthetic, and biologically active compound.

Short Description :

An Agonist of AMPA Receptors

MW :

402.1

Source :

Synthetic

Molecular formula :

C16H16F2N2O4S2.

Effective Concentration :

10 - 250 μM.

Activity :

PEPA is a selective AMPA receptor agonist (allosteric potentiator)1.

Reconstitution and Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity

Solubility :

50 mM in DMSO. Centrifuge all product preparations before use (10000 x g for 1 min).

Storage of solutions :

Up to four weeks at 4°C or three months at -20°C.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>99.9%

CAS No :

141286-78-4

Form :

Lyophilized powder.

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

2-(2,6-difluoro-4-(2-(phenylsulfonamido)ethylthio)phenoxy)acetamide.

more info or order :

Alomone Labs product webpage