chemical

1-Phenylbiguanide hydrochloride

P-190

P-190_1 g

1-Phenylbiguanide hydrochloride

Non Antibodies

S. Molecules

https://www.uniprot.org/uniprotkb/P81862/entry

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Phenylbiguanide (PBG) acts as an agonist of 5-HT3 receptors, with an EC50 value two fold greater than that of 5-HT and was found to imitate the effects of 5-HT on mammalian peripheral neurons. PBG causes dopamine release and has been used in many studies as a selective agonist to arouse reflex bradycardia and hypotension through activation of cardiac and pulmonary vagal afferents1,3.For over 40 years, PBG has been used as a selective agonist to identify cardiac and pulmonary vagal C fibers and the associated reflex bradycardia and depressor responses. It is capable to stimulate cardiac chemosensitive receptors with vagal afferent pathways and the sensory nerves in different regions of the body3.In one study conducted in rats, it was found that phenylbiguanide depolarizes the rat vagus nerve and mimics the effects of 5-HT on the vagus nerve. In addition, it was shown that phenylbiguanide causes reflex falls in heart rate and blood pressure in the cat and induces pain following application to the bases of blisters in human subjects1.It has been documented that right atrial bolus injection of phenylbiguanide can stimulate bronchopulmonary C-fibers that play an important role in modulating the respiratory rhythm to produce apnea2.

Alomone Labs

Serotonin-gated channels

1-Phenylbiguanide hydrochloride (#P-190) is a highly pure, synthetic, and biologically active compound.

A Potent and Selective Agonist of 5-HT3 Receptors

213.67

PBG

Synthetic

C8H12ClN5.

0.1-100 µM.

1-Phenylbiguanide hydrochloride is an activator of 5-HT3 channels1.

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity

25 mM in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).

Up to four weeks at 4°C or three months at -20°C.

1-2 Business Days

Israel/IL

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

>99%

55-57-2

Lyophilized powder.

Contact Alomone Labs for technical support and product customization

41116134

Yes

1-(diaminomethylidene)-2-phenylguanidine;hydrochloride.