β-Pompilidotoxin | Alomone Labs P-180 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

β-Pompilidotoxin

catalog :

P-180

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Radwański P, Ho H, Veeraraghavan R, Brunello L, Liu B, Belevych A, et al. Neuronal Na+ Channels Are Integral Components of Pro-arrhythmic Na+/Ca2+ Signaling Nanodomain That Promotes Cardiac Arrhythmias During β-adrenergic Stimulation. JACC Basic Transl Sci. 2016;1:251-266 pubmed
Gilchrist J, Olivera B, Bosmans F. Animal toxins influence voltage-gated sodium channel function. Handb Exp Pharmacol. 2014;221:203-29 pubmed publisher
Gilchrist J, Das S, Van Petegem F, Bosmans F. Crystallographic insights into sodium-channel modulation by the ?4 subunit. Proc Natl Acad Sci U S A. 2013;110:E5016-24 pubmed publisher

image

image 1 :

Alomone Labs ?-Pompilidotoxin enhances native NaVcurrents in ND7-23 cells. - The effect on inward currents of a bath application of 100 M?-Pompilidotoxin(#P-180) (cyan). NaVcurrents were elicited every 15 sec from a holding potential of -100 mV by 20 ms test pulses to -10 mV.

image 2 :

Western blot analysis of rat dorsal root ganglion lysate: - 1.Anti-NRG1 Type III (extracellular) Antibody (#ANR-113) (1:200).2. Anti-NRG1 Type III (extracellular) Antibody preincubated with the negative control antigen.

product information

cat :

P-180

SKU :

P-180_1 mg

Product Name :

β-Pompilidotoxin

Group Type :

Non Antibodies

Product Type :

Proteins

Accession :

P69392

Accession Number :

https://www.uniprot.org/uniprotkb/P69392/entry

Applications :

Electrophysiology

Formulation :

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Reconstitution and Solubility :

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Storage Before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Origin :

Batozonellus maculifrons (Solitary wasp)

Source :

Synthetic peptide

Gene ID :

SCN1A,SCN2A,SCN3A,SCN8A,SCN9A

Product Page - Scientific background :

β-PMTX was originally isolated from Anoplius samariensis and Batozonellus maclifrons wasps. This is a synthetic version of the peptide.β-PMTX is a homologous peptide to α-PMTX (position 12 is Arginine instead of Lysine). β-PMTX induces repetitive action potentials and facilitates both excitatory and inhibitory synaptic transmission.Recently it was found that this toxin slows the Na+-channel inactivation, without changing the peak current-voltage relationship or the activation time course of the TTX-sensitive Na+ currents in the neuromuscular synapse of the lobster walking leg and in the rat trigeminal ganglion neurons.1-3 β-PMTX is five times more potent than α-PMTX.1,2

Supplier :

Alomone Labs

Target :

TTX-sensitive Na+ channels

Long Description :

An Enhancer of NaV Currents

Short Description :

An Enhancer of NaV Currents

MW :

1558 Da

Synonyms :

β-PMTX

Modifications :

Lys13 - C-terminal amidation

Molecular formula :

C71H124N22O17

Effective Concentration :

10 - 100 µM

Activity :

β-Pompilidotoxin slows NaV channel inactivation, without changing the peak current-voltage relationship or the activation time course of the TTX-sensitive Na+ currents in the neuromuscular synapse1-3. β-Pompilidotoxin is five times more potent than α-Pompilidotoxin1,2.

Storage of solutions :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Purity :

≥98% (HPLC)

CAS No :

216064-36-7

Form :

Lyophilized

Comment :

Contact Alomone Labs for technical support and product customization

Sequence :

RIKIGLFDQLSRL-NH2

Is Toxin :

Yes

UNSPSC :

12352202

Bioassay Tested :

yes

Steril endotoxin free :

more info or order :

Alomone Labs product webpage