chemical

3R12Rα-Pompilidotoxin

P-176

P-176_1 mg

3R12Rα-Pompilidotoxin

Non Antibodies

Proteins

Electrophysiology

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Anoplius samariensis (Solitary wasp)

Synthetic peptide

SCN1A,SCN2A,SCN3A,SCN8A,SCN9A

Pompilidotoxins (PMTX, α and β) are small peptides consisting of 13 amino acids.α-PMTX (#P-170) induces a unique pattern of repetitive action potentials previous seen with other neurotoxins and thus facilitate both excitatory and inhibitory synaptic transmission.Recently it was found that this toxin slows the Na+-channel inactivation, without changing the peak current-voltage relationship or the activation time course of the TTX-sensitive Na+ currents in the neuromuscular synapse of the lobster walking leg and in the rat trigeminal ganglion neurons2-4. Effective at concentrations of 10 nM in cultured rat cortical neurons5.3R12Rα-Pompilidotoxin (3R12Rα-PMTX) is a mutated form of the α-PMTX, originally isolated from the solitary wasp (Anoplius samariensis) venom1 and is a synthetic version of the peptide.3R12RKα-PMTX is mutated at position 3 as well as 12 with Lysine to Arginine transition, which enhanced its activity on voltage-gated Na+ channels 5-fold relative to α-PMTX1,3.

Alomone Labs

NaV Na+ channels

An Activator of NaV Channels

An Activator of NaV Channels

1586 Da

3R12Rα-PMTX

Leu13 - C-terminal amidation

C71H124N24O17

100 - 200 µM

3R12Rα-PMTX slows NaV channel inactivation without changing the peak current-voltage relationship or the activation time course of the TTX-sensitive Na+ currents in the neuromuscular synapse1-3.

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

1-2 Business Days

Israel/IL

≥98% (HPLC)

Lyophilized

Contact Alomone Labs for technical support and product customization

RIRIGLFDQLSRL-NH2

Yes

12352202

yes

no