Okadaic Acid (ammonium salt) | Alomone Labs O-800 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

Okadaic Acid (ammonium salt)

catalog :

O-800

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Potenza D, Janícek R, Fernández Tenorio M, Camors E, Ramos Mondragón R, Valdivia H, et al. Phosphorylation of the ryanodine receptor 2 at serine 2030 is required for a complete β-adrenergic response. J Gen Physiol. 2019;151:131-145 pubmed publisher

image

image 1 :

Alomone Labs Okadaic Acid induces apoptosis in Jurkat cells. - Cells were grown to 70% confluency followed by the addition of 1MOkadaic Acid (ammonium salt)(#O-800) (orOkadaic Acid (sodium salt)(#O-900)) or vehicle for 6 h. At the end of the incubation cell proteins were resolved by SDS-PAGE and probed with anti-Caspase 3 antibody.

image 2 :

Alomone Labs Okadaic Acid inhibits PP2A in 3T3-L1 cells. - Cells were grown to 70% confluence and serum starved for 18 h. Forin vivophosphatase activity the growth medium was replaced with HENK?s and the cells were preincubated in the presence or absence of 100 nMOkadaic Acid (ammonium salt)(#O-800) (orOkadaic Acid (sodium salt)(#O-900)) and 100 nM1-Norokadaone(#N-750) followed by a 20 min incubation with or without 20 M NEM. The cells were then stimulated with 5 ng/ml PDGF for 30 min. At the end of the incubation cell proteins were resolved by SDS-PAGE and probed with anti-phospho-Akt antibodies.

image 3 :

Western blot analysis ofrat brain synaptosomal fraction(lanes 1 and 3) and mousebrain synaptosomal fraction(lanes 2 and 4): - 12. Guinea pigAnti-D1Dopamine ReceptorAntibody (#AGP-100) (1:400).34. Guinea pig Anti-D1Dopamine Receptor Antibody preincubated with the negative control antigen.

product information

CAT :

O-800

SKU :

O-800_0.1 mg

Product Name :

Okadaic Acid (ammonium salt)

Group Type :

Non Antibodies

Product Type :

S. Molecules

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

Okadaic acid is a polyether compound isolated from the black sponge Halichondria okadai. It was previously demonstrated as a non-TPA-type tumor promoter and then as a cell-permeable, potent and specific inhibitor of serine/threonine protein phosphatases 1 and 2A. In higher concentrations Okadaic acid induced apoptosis (IC50 = 0.5 µM).1-4Okadaic acid down-regulates the TNF receptor, induces the release of Ca2+ from intracellular stores in ECV304 endothelial cells and inhibits GH4 cell proliferation in a concentration-dependent manner (IC50 = 5 nM).5,6

Supplier :

Alomone Labs

Target :

Protein Phosphatase 1, Protein Phosphatase 2

Long Description :

Okadaic Acid (ammonium salt) (#O-800) is a highly pure, natural, and biologically active compound.

Short Description :

A Potent Inhibitor of Protein Phosphatase 1 and Protein Phosphatase 2

MW :

822.04

Synonyms :

Halochondrine A

Source :

Natural

Molecular formula :

C44H71O13N.

Effective Concentration :

1 nM-1 µM.

Activity :

Okadaic Acid is a potent inhibitor of PP1 and PP2A1. At higher concentrations, it also promotes apoptosis2.

Reconstitution and Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity

Solubility :

DMSO, ethanol, acetone, or methanol. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions :

Up to one week at 4°C or three months at -20°C.Protect from light.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>98%

CAS No :

155716-06-6

Form :

Lyophilized powder.

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

(2R)-3-[(2S,6R,8S,11R)-2-[(E,2R)-4- [(2S,2'R,4R,4aS,6R,8aR)-4-hydroxy-2-[(1S,3S)-1-hydroxy-3-[(2S,3R,6S)- 3-methyl-1,7-dioxaspiro[5.5]undecan-2-yl]butyl]-3- methylidenespiro[4a,7,8,8a-tetrahydro-4H-pyrano[3,2-b]pyran-6,5'- oxolane]-2'-yl]but-3-en-2-yl]-11-hydroxy-4-methyl-1,7- dioxaspiro[5.5]undec-4-en-8-yl]-2-hydroxy-2-methylpropanoic acid.

more info or order :

Alomone Labs product webpage