4-ONE | Alomone Labs O-145 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

4-ONE

catalog :

O-145

more info or order :

Alomone Labs product webpage

image

image 1 :

Alomone Labs PF-04856264 blocks NaV1.7 channels expressed in Xenopus oocytes. - A. Time course of NaV1.7 current amplitude and inhibition by 500 nMPF-04856264(#P-265). Currents were elicited by application of a 100 ms voltage step to 0 mV (H.P= -70mV to induce inactivation) every 10 seconds. B. Superimposed example traces of current responses before and during perfusion of 500 nM PF-04856264 as indicated.

image 2 :

Alomone Labs 4-ONE activates TRPA1 channels expressed in HEK-293 cells. - Dose response curve of the activation of human TRPA1 channels by increasing concentrations of4-ONE(#O-145). Cells were loaded with Calcium 5 dye. Changes in intracellular Ca2+following 4-ONE application were detected as changes in relative fluorescence (RFU). EC50was determined at 23.1 M.

product information

CAT :

O-145

SKU :

O-145_10 mg

Product Name :

4-ONE

Group Type :

Non Antibodies

Product Type :

S. Molecules

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

4-ONE is a lipid peroxidation product that acts as a TRPA1 (transient receptor potential ankyrin 1) channel activator and displays an EC50 value of 1.9 µM1. The compound contains an electrophilic unsaturated carbonyl moiety and forms adducts with lysine, histidine, and cysteine residues1. 4-ONE is rich in β-sheet structures and is considered to be a reactive aldehyde that may be also cytotoxic2.A study conducted with 4-ONE showed that the compound is able to trigger unilateral mechanical hyperalgesia and vasodilatation in mice and also triggersTRPA1-independent effects that relate to edema formation and pain3.TRPA1 is a non-selective cation channel expressed in the small neurons of the dorsal root and trigeminal ganglia. TRPA1 channels play an important role in models of inflammatory and neuropathic pain and have the ability to detect pungent or irritant compounds4.

Supplier :

Alomone Labs

Target :

TRPA1 channels

Long Description :

4-ONE (#O-145) is a highly pure, synthetic, and biologically active compound.

Short Description :

An Activator of TRPA1 Channel

MW :

154.2

Synonyms :

4-Oxo-2-nonenal

Source :

Synthetic

Molecular formula :

C9H14O2.

Effective Concentration :

1-100 µM.

Activity :

4-ONE (4-Oxo-2-nonenal) is a lipid peroxidation product with multiple biological activities, shown to activate TRPA1 channels (EC50 = 1.9 µM)1.

Reconstitution and Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. Repeat freeze-thawing may result in loss of activity.

Solubility :

Supplied in oil form. Soluble in DMSO, methanol, ethanol.Centrifuge all products before use (10000 x g 5 min).

Storage of solutions :

-20°C.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>97%

CAS No :

103560-62-9

Form :

Lyophilized powder.

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

(E)-4-oxonon-2-enal.

more info or order :

Alomone Labs product webpage