chemical

Optovin

O-140

O-140_10 mg

Optovin

Non Antibodies

S. Molecules

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Optovin, a novel neuroactive small molecule, acts as a photosensitive TRPA1 (transient receptor potential ankyrin 1) channel activator win EC50 values of 2 µM1. Optovin is a rhodanine derivative and activates TRPA1 channels in vitro and facilitates repeated photoactivation of motor behaviors in vivo1.In humans, Optovin activates TRPA1 channels through structure-dependent photochemical reactions with redox-sensitive cysteine residues. In animals with severed spinal cords Optovin enables control of motor activity in the paralyzed extremities by localized illumination1.TRPA1 channels are non-selective cation channels expressed in the small neurons of the dorsal root and trigeminal ganglia. They play an important role in models of inflammatory and neuropathic pain and have the ability to detect pungent or irritant compounds2.

Alomone Labs

TRPA1 channels

Optovin (#O-140) is a highly pure, synthetic, and biologically active compound.

A Novel Photosensitive Activator of TRPA1 Channel

315.41

Synthetic

C15H13N3OS2.

10-100 µM.

Optovin is a photosensitive TRPA1 ligand that activates TRPA1 channels in vitro and facilitates repeated photoactivation of motor behaviors in vivo (EC50 = 2 µM) when reversibly activated by violet light (405 nm)1.

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. Repeat freeze-thawing may result in loss of activity.

Soluble in DMSO.Centrifuge all products before use (10000 x g 5 min).

Up to three months at -20°C.

1-2 Business Days

Israel/IL

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

>98%

348575-88-2

Lyophilized powder.

Contact Alomone Labs for technical support and product customization

41116134

Yes

(5E)-5-[(2,5-dimethyl-1-pyridin-3-ylpyrrol-3-yl)methylidene]-2-sulfanylidene-1,3-thiazolidin-4-one.